Literature summary for 4.6.1.1 extracted from

Wang, S.C.; Lai, H.L.; Chiu, Y.T.; Ou, R.; Huang, C.L.; Chern, Y.
Regulation of type V adenylate cyclase by Ric8a, a guanine nucleotide exchange factor (2007), Biochem. J., 406, 383-388.

Search for more literature for 4.6.1.1

Activating Compound

Activating Compound	Comment	Organism	Structure
forskolin	-	Rattus norvegicus
isoprenaline	-	Rattus norvegicus

Application

Application	Comment	Organism
additional information	interaction between the N-terminus of AC5 and the guanine nucleotide exchange factor Ric8a provides a pathway to fine-tune AC5 activity via a Galphai mediated pathway	Rattus norvegicus

Cloned(Commentary)

Cloned (Comment)	Organism
Escherichia coli BL21 (DE3) transformed with pET11D-AC5N1-215, AC5-myc and HA-Ric8a expressed in HEK-293T cells	Rattus norvegicus

Inhibitors

Inhibitors	Comment	Organism	Structure
Galphai2G203T	chronic treatment of HEK-293T cells reduces AC5 activity	Rattus norvegicus
pertussis toxin	chronic treatment of HEK-293T cells reduces AC5 activity	Rattus norvegicus
Ric8a	i.e., resistance to inhibitors of cholinesterase 8 homolog A, AC5 interacts with Ric8a through directly interacting at its N-terminus, selectively suppresses AC5 activity, does not further suppress AC5s activity in the presence of somatostatin, effectively suppresses the forskolin-stimulated activity of AC5, but not that of AC6. Treating cells with pertussis toxin or expressing a dominant negative Galphai mutant abolishes the suppressive effect, markedly suppresses the activation of AC5 induced by isoprenaline of a Galphas coupled receptor in an isoform-specific manner, while the isoprenaline-evoked activity of AC6 is not affected	Rattus norvegicus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Rattus norvegicus	16020	-

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	Q04400	Sprague-Dawley rats	-

Purification (Commentary)

Purification (Comment)	Organism
by Ni-coupled beads	Rattus norvegicus

Source Tissue

Source Tissue	Comment	Organism	Textmining
brain	-	Rattus norvegicus	-
embryo	-	Rattus norvegicus	-
additional information	AC5 highly enriched in the striatum	Rattus norvegicus	-

Synonyms

Synonyms	Comment	Organism
AC5	-	Rattus norvegicus
AC6	-	Rattus norvegicus
type V adenylate cyclase	-	Rattus norvegicus

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Release 2023.1 (January 2023)