Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bacillus anthracis |
Crystallization (Comment) | Organism |
---|---|
3.2 A resolution | Bacillus anthracis |
Protein Variants | Comment | Organism |
---|---|---|
K372A | mutant with 30fold reduced catalytic rate constant and 3fold increased Km value for ATP | Bacillus anthracis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cAMP | mutant K372A shows at least 20fold increase in the IC50 value | Bacillus anthracis | |
diphosphate | no difference between wild type and mutant K372A | Bacillus anthracis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
ATP | 30°C, pH 7.2, 2 microM free Ca2+ | Bacillus anthracis | |
1.3 | - |
ATP | 30°C, pH 7.2, 0.1 microM free Ca2+ | Bacillus anthracis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Bacillus anthracis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus anthracis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus anthracis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
Vmax forward reaction 1200/s, pH 7.2, 30°C, 0.1 microM free Ca2+, 147/s pH 7.2, 30°C, 2 microM free Ca2+ | Bacillus anthracis |
additional information | - |
Vmax reverse reaction 2000/s, pH 7.2, 30°C, 0.1 microM free Ca2+, 100-300/s pH 7.2, 30°C, 2 microM free Ca2+ | Bacillus anthracis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP | - |
Bacillus anthracis | cAMP + diphosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
adenylyl cyclase | - |
Bacillus anthracis |
Edema factor | - |
Bacillus anthracis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
diphosphate | forward reaction, 2 microM free Ca2+, pH 7.2, 30°C | Bacillus anthracis | |
0.3 | - |
diphosphate | forward reaction, 0.1 microM free Ca2+, pH 7.2, 30°C | Bacillus anthracis | |
1 | - |
diphosphate | reverse reaction, 0.1 microM free Ca2+, pH 7.2, 30°C | Bacillus anthracis | |
1.2 | - |
diphosphate | reverse reaction, 2 microM free Ca2+, pH 7.2, 30°C | Bacillus anthracis | |
1.3 | - |
cAMP | forward reaction, 2 microM free Ca2+, pH 7.2, 30°C | Bacillus anthracis | |
1.5 | - |
cAMP | reverse reaction, 0.1 microM free Ca2+, pH 7.2, 30°C | Bacillus anthracis | |
2 | - |
cAMP | reverse reaction, 2 microM free Ca2+, pH 7.2, 30°C | Bacillus anthracis | |
3.7 | - |
cAMP | forward reaction, 0.1 microM free Ca2+, pH 7.2, 30°C | Bacillus anthracis |