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Literature summary for 4.6.1.1 extracted from

  • Guo, Q.; Shen, Y.; Zhukovskaya, N.L.; Florian, J.; Tang, W.J.
    Structural and Kinetic Analyses of the Interaction of Anthrax Adenylyl Cyclase Toxin with Reaction Products cAMP and Pyrophosphate (2004), J. Biol. Chem., 279, 29427-29435.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bacillus anthracis

Crystallization (Commentary)

Crystallization (Comment) Organism
3.2 A resolution Bacillus anthracis

Protein Variants

Protein Variants Comment Organism
K372A mutant with 30fold reduced catalytic rate constant and 3fold increased Km value for ATP Bacillus anthracis

Inhibitors

Inhibitors Comment Organism Structure
cAMP mutant K372A shows at least 20fold increase in the IC50 value Bacillus anthracis
diphosphate no difference between wild type and mutant K372A Bacillus anthracis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
ATP 30°C, pH 7.2, 2 microM free Ca2+ Bacillus anthracis
1.3
-
ATP 30°C, pH 7.2, 0.1 microM free Ca2+ Bacillus anthracis

Localization

Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Bacillus anthracis

Organism

Organism UniProt Comment Textmining
Bacillus anthracis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bacillus anthracis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
Vmax forward reaction 1200/s, pH 7.2, 30°C, 0.1 microM free Ca2+, 147/s pH 7.2, 30°C, 2 microM free Ca2+ Bacillus anthracis
additional information
-
Vmax reverse reaction 2000/s, pH 7.2, 30°C, 0.1 microM free Ca2+, 100-300/s pH 7.2, 30°C, 2 microM free Ca2+ Bacillus anthracis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP
-
Bacillus anthracis cAMP + diphosphate
-
r

Synonyms

Synonyms Comment Organism
adenylyl cyclase
-
Bacillus anthracis
Edema factor
-
Bacillus anthracis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.06
-
diphosphate forward reaction, 2 microM free Ca2+, pH 7.2, 30°C Bacillus anthracis
0.3
-
diphosphate forward reaction, 0.1 microM free Ca2+, pH 7.2, 30°C Bacillus anthracis
1
-
diphosphate reverse reaction, 0.1 microM free Ca2+, pH 7.2, 30°C Bacillus anthracis
1.2
-
diphosphate reverse reaction, 2 microM free Ca2+, pH 7.2, 30°C Bacillus anthracis
1.3
-
cAMP forward reaction, 2 microM free Ca2+, pH 7.2, 30°C Bacillus anthracis
1.5
-
cAMP reverse reaction, 0.1 microM free Ca2+, pH 7.2, 30°C Bacillus anthracis
2
-
cAMP reverse reaction, 2 microM free Ca2+, pH 7.2, 30°C Bacillus anthracis
3.7
-
cAMP forward reaction, 0.1 microM free Ca2+, pH 7.2, 30°C Bacillus anthracis