Literature summary for 4.6.1.1 extracted from

Reddy, S.K.; Kamireddi, M.; Dhanireddy, K.; Young, L.; Davis, A.; Reddy, P.T.
Eukaryotic-like adenylyl cyclases in Mycobacterium tuberculosis H37Rv: cloning and characterization (2001), J. Biol. Chem., 276, 35141-35149.

Search for more literature for 4.6.1.1

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Mycobacterium tuberculosis

Protein Variants

Protein Variants	Comment	Organism
R18A	88% of wild-type activity, in the presence of 2 mM Mn2+	Mycobacterium tuberculosis
R18A/R19G	67% of wild-type activity, in the presence of 2 mM Mn2+	Mycobacterium tuberculosis
R19G	78% of wild-type activity, in the presence of 2 mM Mn2+	Mycobacterium tuberculosis
R27A	78% of wild-type activity, in the presence of 2 mM Mn2+	Mycobacterium tuberculosis
R31G	118% of wild-type activity, in the presence of 2 mM Mn2+	Mycobacterium tuberculosis
R43A	61% of wild-type activity, in the presence of 2 mM Mn2+	Mycobacterium tuberculosis
R43A/R44G	1.3% of wild-type activity, in the presence of 2 mM Mn2+	Mycobacterium tuberculosis
R43K	77% of wild-type activity, in the presence of 2 mM Mn2+	Mycobacterium tuberculosis
R43K/R44K	39% of wild-type activity, in the presence of 2 mM Mn2+	Mycobacterium tuberculosis
R44G	41% of wild-type activity, in the presence of 2 mM Mn2+	Mycobacterium tuberculosis
R44K	116% of wild-type activity, in the presence of 2 mM Mn2+	Mycobacterium tuberculosis
R46A	85% of wild-type activity, in the presence of 2 mM Mn2+	Mycobacterium tuberculosis
R46K	135% of wild-type activity, in the presence of 2 mM Mn2+	Mycobacterium tuberculosis
R4G	77% of wild-type activity, in the presence of 2 mM Mn2+	Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.05	-	ATP	recombinant adenylyl cclase, in the presence of Mn2+	Mycobacterium tuberculosis
3.5	-	ATP	recombinant adenylyl cclase, in the presence of Mg2+	Mycobacterium tuberculosis

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	six putative helical transmembrane domains	Mycobacterium tuberculosis	16020	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mn2+	1 mM, 47fold activation	Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
45000	-	x * 45000, SDS-PAGE	Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP	Mycobacterium tuberculosis	-	3',5'-cyclic AMP + diphosphate	-	?
ATP	Mycobacterium tuberculosis H37Rv	-	3',5'-cyclic AMP + diphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	P9WQ35	-	-
Mycobacterium tuberculosis H37Rv	P9WQ35	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant adenylyl cyclase	Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.48	-	in the presence of physiological ATP concentrations and 1 mM Mn2+	Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP	-	Mycobacterium tuberculosis	3',5'-cyclic AMP + diphosphate	-	?
ATP	-	Mycobacterium tuberculosis H37Rv	3',5'-cyclic AMP + diphosphate	-	?

Subunits

Subunits	Comment	Organism
?	x * 45000, SDS-PAGE	Mycobacterium tuberculosis

Synonyms

Synonyms	Comment	Organism
adenylyl cyclase	-	Mycobacterium tuberculosis
Rv1625c	-	Mycobacterium tuberculosis

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Release 2023.1 (January 2023)