Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.6.1.1 extracted from

  • Kanacher, T.; Schultz, A.; Linder, J.U.; Schultz, J.E.
    A GAF-domain-regulated adenylyl cyclase from Anabaena is a self-activating cAMP switch (2002), EMBO J., 21, 3672-3680.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
cAMP activation of recombinant cyaB1 via N-terminal GAF domains Anabaena sp.

Cloned(Commentary)

Cloned (Comment) Organism
expression of cyaB1-1-859 AC holoenzyme and catalytic domain cyaB1-595-859 in Escherichia coli Anabaena sp.

Protein Variants

Protein Variants Comment Organism
D650A mutation in cyaB1-595-859 catalytical domain, no activity Anabaena sp.
N728A mutation in cyaB1-595-859 catalytical domain, no activity Anabaena sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
ATP 37°C, pH 7.5, recombinant cyaB1 AC, in the presence of 2 mM Mn2+ and 0.1 mM cAMP Anabaena sp.
0.011
-
ATP 37°C, pH 7.5, recombinant cyaB1 AC, in the presence of 2 mM Mn2+ Anabaena sp.
0.024
-
ATP 37°C, pH 7.5, recombinant cyaB1 AC, in the presence of 10 mM Mg2+ and 0.1 mM cAMP Anabaena sp.
0.038
-
ATP 37°C, pH 7.5, recombinant cyaB1 AC, in the presence of 10 mM Mg2+ Anabaena sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Anabaena sp. 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ supports activity of recombinant cyaB1 AC better than Mn2+ Anabaena sp.
Mn2+ activation of recombinant cyaB1 AC Anabaena sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP Anabaena sp.
-
3',5'-cyclic AMP + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Anabaena sp. P94181 PCC 7120, class III type adeylyl caclase
-

Purification (Commentary)

Purification (Comment) Organism
recombinant cyaB1 Anabaena sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP
-
Anabaena sp. 3',5'-cyclic AMP + diphosphate
-
?

Synonyms

Synonyms Comment Organism
AC
-
Anabaena sp.
adenylyl cyclase
-
Anabaena sp.
cyaB1
-
Anabaena sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
recombinant cyaB1 AC holoenzyme Anabaena sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
recombinant cyaB1 AC holoenzyme Anabaena sp.