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Literature summary for 4.6.1.1 extracted from

  • Bieger, B.; Essen, L.O.
    Crystallization and preliminary X-ray analysis of the catalytic domain of the adenylate cyclase GRESAG4.1 from Trypanosoma brucei (2000), Acta Crystallogr. Sect. D, 56, 359-362.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Trypanosoma brucei

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion, 0.001 ml protein solution containing 4 mg/ml GRESAG4.1 in 5 mM Tris-HCl, pH 8.0 is mixed with either 1.8-1.9 M ammonium sulfate, pH 6.5 and pH 7.75 or 1.2 M sodium citrate at pH 5.4, crystals diffract to 1.9-2.1 A resolution Trypanosoma brucei

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Trypanosoma brucei 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP Trypanosoma brucei
-
3',5'-cyclic AMP + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GRESAG4.1 Trypanosoma brucei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP
-
Trypanosoma brucei 3',5'-cyclic AMP + diphosphate
-
?

Synonyms

Synonyms Comment Organism
adenylyl cyclase
-
Trypanosoma brucei
GRESAG4.1
-
Trypanosoma brucei