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Literature summary for 4.5.1.B1 extracted from
- Isolation of a novel thermostable dehydrochlorinase (LinA) from a soil metagenome (2011), 3 Biotech, 1, 193-198.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene linA, DNA and amino acid sequence determination and analysis, sequence comparison, subcloning in Escherichia coli strain DH5alpha, expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) | uncultured bacterium |
| gene linA-UT26, sequence comparison | Sphingomonas paucimobilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sphingomonas paucimobilis | P51697 | LinA-type 1, sequence of LinA-type1, B5ANV6, is 100% identical to LinA-UT26; i.e. Pseudomonas paucimobilis, gene linA-UT26 | - |
| uncultured bacterium | B5ANU3 | LinA-type 2; isolated from soil, gene linA | - |
| uncultured bacterium | B5ANV6 | LinA-type 1, sequence of LinA-type1 is 100% identical to LinA-UT26, P51697; isolated from soil, gene linA | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant C-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | uncultured bacterium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,3,4,5,6-pentachlorocyclohexene | - |
Sphingomonas paucimobilis | 1,3,4,6-tetrachloro-1,4-cyclohexadiene + HCl | - |
? |  |
| 1,3,4,5,6-pentachlorocyclohexene | - |
uncultured bacterium | 1,3,4,6-tetrachloro-1,4-cyclohexadiene + HCl | - |
? | |
| gamma-hexachlorocyclohexane | activity differs with isomers alpha, gamma, and delta, for LinA type 1 the activity is highest with the alpha isomer, no activity with the beta isomer | Sphingomonas paucimobilis | pentachlorocyclohexene + HCl | - |
? | |
| gamma-hexachlorocyclohexane | activity differs with isomers alpha, gamma, and delta, for LinA type 1 the activity is highest with the gamma-isomer, no activity with the beta isomer | uncultured bacterium | pentachlorocyclohexene + HCl | - |
? | |
| gamma-hexachlorocyclohexane | activity differs with isomers alpha, gamma, and delta, for LinA type 2 the activity is highest with the alpha-isomer, no activity with the beta isomer | uncultured bacterium | pentachlorocyclohexene + HCl | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| LinA | - |
uncultured bacterium |
| LinA-type1 | - |
uncultured bacterium |
| LinA-type2 | - |
uncultured bacterium |
| LinA-UT26 | - |
Sphingomonas paucimobilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Sphingomonas paucimobilis |
| 35 | - |
- |
uncultured bacterium |
| 70 | - |
- |
uncultured bacterium |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 65 | activity range, rapid drop in activity above 60°C, profile overview | uncultured bacterium |
| 30 | 75 | activity range, rapid drop in activity above 70°C, profile overview | uncultured bacterium |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
loss of 50% activity after 60 min | uncultured bacterium |
| 45 | - |
Tm, complete inactivation after 1 h | Sphingomonas paucimobilis |
| 45 | 65 | 8 h, completely stable, Tm is 65°C | uncultured bacterium |
| 70 | - |
3 min, inactivation | Sphingomonas paucimobilis |
| 70 | - |
3 min, inactivation | uncultured bacterium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Sphingomonas paucimobilis |
| 8 | - |
assay at | uncultured bacterium |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the enzyme mediates the first step of aerobic degradation of a chlorinated insecticide gamma-hexachlorocyclohexane | Sphingomonas paucimobilis |
| metabolism | the enzyme mediates the first step of aerobic degradation of a chlorinated insecticide gamma-hexachlorocyclohexane | uncultured bacterium |
| additional information | LinA-type2 differs from archetypal LinA-UT26 by ten residues i.e. K20Q, A23G, F68Y, C71T, L96C, F113Y, D115 N, R129L, A131G and T133 M | Sphingomonas paucimobilis |
| additional information | LinA-type2 differs from archetypal LinA-UT26 by ten residues i.e., K20Q, A23G, F68Y, C71T, L96C, F113Y, D115 N, R129L, A131G and T133 M | uncultured bacterium |
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