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Literature summary for 4.5.1.3 extracted from

  • Vuilleumier, S.; Sorribas, H.; Leisinger, T.
    Identification of a novel determinant of glutathione affinity in dichloromethane dehalogenases/glutathione S-transferases (1997), Biochem. Biophys. Res. Commun., 238, 452-456.
    View publication on PubMed
Search for more literature for 4.5.1.3

Activating Compound

Activating Compound Comment Organism Structure
glutathione KM: 0.066 for wild-type, KM: 0.091 for V18T, KM: 0.112 for V18A and KM: 0.456 for V18I Methylophilus sp.
glutathione KM: 0.174 Hyphomicrobium sp.
glutathione KM: 0.403 Methylorubrum extorquens

Cloned(Commentary)

Cloned (Comment) Organism
-
 Methylophilus sp.

Protein Variants

Protein Variants Comment Organism
V18A lower affinity for GSH and slower turnover of dichlormethane than wild-type Methylophilus sp.
V18I lower affinity for GSH and slower turnover of dichlormethane than wild-type Methylophilus sp.
V18T lower affinity for GSH and slower turnover of dichlormethane than wild-type Methylophilus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.009
-
 Dichloromethane pH 8 Methylorubrum extorquens
0.011
-
 Dichloromethane pH 8 Hyphomicrobium sp.
0.04
-
 Dichloromethane pH 8.5, V18I Methylophilus sp.
0.041
-
 Dichloromethane pH 8.5, V18A Methylophilus sp.
0.059
-
 Dichloromethane pH 8.5, wild-type Methylophilus sp.
0.06
-
 Dichloromethane pH 8.5, V18T Methylophilus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dichloromethane + H2O Hyphomicrobium sp.
-
 formaldehyde + Cl-
-
 ?
dichloromethane + H2O Methylorubrum extorquens
-
 formaldehyde + Cl-
-
 ?
dichloromethane + H2O Methylophilus sp.
-
 formaldehyde + Cl-
-
 ?
dichloromethane + H2O Methylophilus sp. DM11
-
 formaldehyde + Cl-
-
 ?

Organism

Organism UniProt Comment Textmining
Hyphomicrobium sp.
-
-
-
Methylophilus sp. P43387
-
-
Methylophilus sp. DM11 P43387
-
-
Methylorubrum extorquens P21161
-
-

Purification (Commentary)

Purification (Comment) Organism
strain DM11 Methylophilus sp.
strain DM2 Hyphomicrobium sp.
strain DM4 Methylorubrum extorquens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dichloromethane + H2O
-
 Hyphomicrobium sp. formaldehyde + Cl-
-
 ?
dichloromethane + H2O
-
 Methylorubrum extorquens formaldehyde + Cl-
-
 ?
dichloromethane + H2O
-
 Methylophilus sp. formaldehyde + Cl-
-
 ?
dichloromethane + H2O
-
 Methylophilus sp. DM11 formaldehyde + Cl-
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.6
-
 Dichloromethane pH 8 Hyphomicrobium sp.
0.6
-
 Dichloromethane pH 8 Methylorubrum extorquens
1.8
-
 Dichloromethane pH 8.5, V18I Methylophilus sp.
2.2
-
 Dichloromethane pH 8.5, V18T and V18A Methylophilus sp.
3.3
-
 Dichloromethane pH 8.5, wild-type Methylophilus sp.