Literature summary for 4.5.1.3 extracted from

Gisi, D.; Willi, L.; Traber, H.; Leisinger, T.; Vuilleumier, S.
Effects of bacterial host and dichloromethane dehalogenase on the competitiveness of methylotrophic bacteria growing with dichloromethane (1998), Appl. Environ. Microbiol., 64, 1194-1202.

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Cloned(Commentary)

Cloned (Comment)	Organism
-	Methylophilus sp.
expression in Escherichia coli	Methylorubrum extorquens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.009	-	Dichloromethane	-	Methylorubrum extorquens
0.059	-	Dichloromethane	-	Methylophilus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
dichloromethane + H2O	Methylorubrum extorquens	-	formaldehyde + Cl-	-	?
dichloromethane + H2O	Methylophilus sp.	-	formaldehyde + Cl-	-	?
dichloromethane + H2O	Methylophilus sp. DM11	-	formaldehyde + Cl-	-	?

Organism

Organism	UniProt	Comment	Textmining
Methylophilus sp.	P43387	-	-
Methylophilus sp. DM11	P43387	-	-
Methylorubrum extorquens	P21161	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
dichloromethane + H2O	-	Methylorubrum extorquens	formaldehyde + Cl-	-	?
dichloromethane + H2O	-	Methylophilus sp.	formaldehyde + Cl-	-	?
dichloromethane + H2O	-	Methylophilus sp. DM11	formaldehyde + Cl-	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.6	-	Dichloromethane	-	Methylorubrum extorquens
3.3	-	Dichloromethane	-	Methylophilus sp.

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Release 2023.1 (January 2023)