Activating Compound | Comment | Organism | Structure |
---|---|---|---|
glutathione | required | Hyphomicrobium sp. | |
glutathione | Km: 0.32 mM | Hyphomicrobium sp. | |
glutathione | sulfhydryl compounds such as 2-mercaptoethanol, DTT or cysteine cannot substitute for reduced glutathione | Hyphomicrobium sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,2-Dichloroethane | - |
Hyphomicrobium sp. | |
1-Chloropropane | - |
Hyphomicrobium sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
Diiodomethane | 30°C, pH 8.2 | Hyphomicrobium sp. | |
0.013 | - |
Dibromomethane | 30°C, pH 8.2 | Hyphomicrobium sp. | |
0.015 | - |
CH2BrCl | 30°C, pH 8.2 | Hyphomicrobium sp. | |
0.03 | - |
Dichloromethane | 30°C, pH 8.2 | Hyphomicrobium sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
alpha6, 6 * 33000, SDS-PAGE | Hyphomicrobium sp. |
195000 | - |
gel filtration | Hyphomicrobium sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dichloromethane + H2O | Hyphomicrobium sp. | - |
formaldehyde + Cl- | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hyphomicrobium sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
strain DM2 | Hyphomicrobium sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.041 | - |
hydroxylapatite eluate | Hyphomicrobium sp. |
Storage Stability | Organism |
---|---|
-20°C, protein concentration 4 mg/ml, 100 mM potassium phosphate, pH 7.5, 2.5 mM DTT, 0.1 mM EDTA, 25% v/v glycerol, stable for 6 months | Hyphomicrobium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CH2BrCl + H2O | - |
Hyphomicrobium sp. | formaldehyde + Br- + Cl- | - |
? | |
dibromomethane + H2O | - |
Hyphomicrobium sp. | formaldehyde + Br- | - |
? | |
dichloromethane + H2O | - |
Hyphomicrobium sp. | formaldehyde + Cl- | - |
? | |
diiodomethane + H2O | - |
Hyphomicrobium sp. | formaldehyde + I- | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | alpha6, 6 * 33000, SDS-PAGE | Hyphomicrobium sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Hyphomicrobium sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
6 days, 50% loss of activity | Hyphomicrobium sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Hyphomicrobium sp. |