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Literature summary for 4.4.1.9 extracted from
- Heterologous expression analyses of rice OsCAS in Arabidopsis and in yeast provide evidence for its roles in cyanide detoxification rather than in cysteine synthesis in vivo (2009), J. Exp. Bot., 60, 993-1008.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2,4-D | activates CAS expression at 10 mM, 2,4-D is a synthetic auxin | Oryza sativa |  |
| auxin | upregulates CAS expression | Oryza sativa | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Arabidopsis thaliana and in Saccharomyces cerevisiae | Oryza sativa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| cyanide | - |
Oryza sativa | |
| ethylene | down-regulates CAS expression in etiolated rice seedlings | Oryza sativa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.27 | - |
HCN | wild type enzyme | Oryza sativa | |
| 0.31 | - |
HCN | recombinant enzyme from Saccharomyces cerevisiae | Oryza sativa | |
| 0.67 | - |
HCN | recombinant enzyme from Arabidopsis thaliana | Oryza sativa | |
| 0.84 | - |
L-cysteine | wild type enzyme | Oryza sativa | |
| 0.91 | - |
L-cysteine | recombinant enzyme from Saccharomyces cerevisiae | Oryza sativa | |
| 1.07 | - |
L-cysteine | recombinant enzyme from Arabidopsis thaliana | Oryza sativa | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Oryza sativa | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
2 * 40000, SDS-PAGE | Oryza sativa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryza sativa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| anti-GFP microbead immunopurification and Mono Q colum chromatography | Oryza sativa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | - |
Oryza sativa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 13.3 | - |
wild type enzyme, after 750fold purification | Oryza sativa |
| 111.3 | - |
recombinant enzyme from Saccharomyces cerevisiae, after 750fold purification | Oryza sativa |
| 181 | - |
recombinant enzyme from Arabidopsis thaliana, after 750fold purification | Oryza sativa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-cysteine + HCN | - |
Oryza sativa | sulfide + L-3-cyanoalanine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 40000, SDS-PAGE | Oryza sativa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-cyanoalanine synthase | - |
Oryza sativa |
| CAS | - |
Oryza sativa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Oryza sativa | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 20 | - |
recombinant enzyme from Arabidopsis thaliana | Oryza sativa | cyanide | |
| 40 | - |
recombinant enzyme from Saccharomyces cerevisiae | Oryza sativa | cyanide | |
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