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Literature summary for 4.4.1.9 extracted from

  • Omura, H.; Kuroda, M.; Kobayashi, M.; Shimizu, S.; Yoshida, T.; Nagasawa, T.
    Purification, characterization and gene cloning of thermostable O-acetyl-L-serine sulfhydrylase forming b-cyano-L-alanine (2003), J. Biosci. Bioeng., 95, 470-475.
    View publication on PubMed
Search for more literature for 4.4.1.9

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
 2 * 34000, SDS-PAGE Geobacillus stearothermophilus
70000
-
 gel filtration Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-cysteine + HCN Geobacillus stearothermophilus
-
 sulfide + L-3-cyanoalanine
-
 ?
O-acetyl-L-serine + CN- Geobacillus stearothermophilus
-
 acetate + L-3-cyanoalanine
-
 ?

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus Q84IF9
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, ethanol, heat, DEAE-Cellulofine, gel filtration, Phenyl-Sepharose Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
483
-
-
 Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-cysteine + HCN
-
 Geobacillus stearothermophilus sulfide + L-3-cyanoalanine
-
 ?
O-acetyl-L-serine + benzylmercaptan
-
 Geobacillus stearothermophilus S-benzyl-L-cysteine + acetate
-
 ?
O-acetyl-L-serine + CN-
-
 Geobacillus stearothermophilus acetate + L-3-cyanoalanine
-
 ?
O-acetyl-L-serine + ethylmercaptan
-
 Geobacillus stearothermophilus S-ethyl-L-cysteine + acetate
-
 ?
O-acetyl-L-serine + methylmercaptan
-
 Geobacillus stearothermophilus S-methyl-L-cysteine + acetate
-
 ?
O-acetyl-L-serine + phenol
-
 Geobacillus stearothermophilus S-phenyl-L-serine + acetate
-
 ?
O-acetyl-L-serine + phenylmercaptan
-
 Geobacillus stearothermophilus S-phenyl-L-cysteine + acetate
-
 ?
O-acetyl-L-serine + propylmercaptan
-
 Geobacillus stearothermophilus S-propyl-L-cysteine + acetate
-
 ?
O-acetyl-L-serine + sulfide 970% of activity with potassium cyanide Geobacillus stearothermophilus L-cysteine + acetate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 34000, SDS-PAGE Geobacillus stearothermophilus

Synonyms

Synonyms Comment Organism
O-acetyl-L-serine sulfhydrylase
-
 Geobacillus stearothermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Geobacillus stearothermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 stable for 30 min at pH 6.0-10.0 Geobacillus stearothermophilus
70
-
 no loss of activity after 30 min at pH 7.5 Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Geobacillus stearothermophilus

pH Range

pH Minimum pH Maximum Comment Organism
4.5 10.5 approx. 50% of maximal activity at pH 4.5 and pH 10.5, respectively Geobacillus stearothermophilus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Geobacillus stearothermophilus