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Literature summary for 4.4.1.5 extracted from
- Inhibition by active site directed covalent modification of human glyoxalase I (2014), Bioorg. Med. Chem., 22, 3301-3308.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression in Escherichia coli strain JM103 | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-bromoacetoxy-1-(S-glutathionyl)-acetoxy butane | competitive inhibition of GLO1, the inhibitor is able to covalently bind to the free sulfhydryl group of Cys60 in the hydrophobic binding pocket adjacent to the enzyme active site and partially inactivate the enzyme, no complete inhibition, binding structure analysis, overview | Homo sapiens |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.182 | - |
methylglyoxal | recombinant enzyme, pH 7.3, 25°C | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 20647 | - |
2 * 20647, sequence calculation, 2 * 21000, recombinant enzyme, SDS-PAGE | Homo sapiens |
| 21000 | - |
2 * 20647, sequence calculation, 2 * 21000, recombinant enzyme, SDS-PAGE | Homo sapiens |
| 42000 | - |
reombinant enzyme, native PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione + methylglyoxal | Homo sapiens | - |
(R)-S-lactoylglutathione | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli strain JM103 by S-hexylglutathione affinity chromatography and gel filtration | Homo sapiens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1580 | - |
purified recombinant enzyme, pH 7.3, 25°C | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione + methylglyoxal | - |
Homo sapiens | (R)-S-lactoylglutathione | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 20647, sequence calculation, 2 * 21000, recombinant enzyme, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Glo1 | - |
Homo sapiens |
| glyoxalase I | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1088 | - |
methylglyoxal | recombinant enzyme, pH 7.3, 25°C | Homo sapiens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.3 | 7.4 | assay at | Homo sapiens |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | glyoxalase I is the first enzyme of the methylglyoxal detoxification pathway | Homo sapiens |
| physiological function | the glyoxalase pathway is responsible for conversion of cytotoxic methylglyoxal to D-lactate. Methylglyoxal toxicity arises from its ability to form advanced glycation end products on proteins, lipids and DNA | Homo sapiens |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.006 | - |
methylglyoxal | recombinant enzyme, pH 7.3, 25°C | Homo sapiens | |
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