Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.4.1.5 extracted from

  • Greig, N.; Wyllie, S.; Patterson, S.; Fairlamb, A.H.
    A comparative study of methylglyoxal metabolism in trypanosomatids (2009), FEBS J., 276, 376-386.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.4.1.5

Cloned(Commentary)

Cloned (Comment) Organism
Trypanosoma brucei lacks GLO1 activity. GLO1 of Trypanosoma cruzi is overexpressed in Trypanosoma brucei in the procyclic and the bloodstream form to complete the glyoxalase system, resulting in an increased resistance to methylglyoxal and increased conversion of methylglyoxal to D-lactate, demonstrating that glyoxalase II (GLO2, EC 3.1.2.6) is functional in vivo Trypanosoma cruzi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
16000
-
 Western blot analysis of whole cell lysate, equivalent to the predicted molecular mass Leishmania major
16000
-
 Western blot analysis of whole cell lysate, equivalent to the predicted molecular mass. Recombinant protein containing a tetracycline-inducible pLew100-TcGLO1 construct, expression of GLO1 of Trypanosoma cruzi in Trypanosoma brucei, Western blot analysis Trypanosoma cruzi

Organism

Organism UniProt Comment Textmining
Leishmania major
-
-
-
no activity in Trypanosoma brucei
-
 overexpression of glyoxalase I from Trypanosomas cruzi exhibits enzyme activity in Trypanosomas brucei. The wild-type Trypansoma brucei lacks the GLO1 activity. No apparent GLO1 gene can be identified in the genome
-
Trypanosoma cruzi
-
-
-

Reaction

Reaction Comment Organism Reaction ID
(R)-S-lactoylglutathione = glutathione + 2-oxopropanal comparative study of methylgloxal metabolism in trypanosomatids: Trypanosoma brucei, Trypanosoma cruzi und Leishmania major. Trypanosoma brucei lacks GLO1 activity. Overexpression of GLO1 from Trypanosoma cruzi in Trypanosoma brucei results in restoration of the glyoxalase system resulting in an increased resistance to methylglyoxal and increased conversion of methylglyoxal to D-lactate, demonstratong that glyoxalase II (GLO2, EC 3.1.2.6) is functional in vivo Trypanosoma cruzi
a
(R)-S-lactoylglutathione = glutathione + 2-oxopropanal comparative study of methylgloxal metabolism in trypanosomatids: Trypanosoma brucei, Trypanosoma cruzi und Leishmania major. Trypanosoma brucei lacks GLO1 activity. Overexpression of GLO1 from Trypanosoma cruzi in Trypanosoma brucei results in restoration of the glyoxalase system resulting in an increased resistance to methylglyoxal and increased conversion of methylglyoxal to D-lactate, demonstratong that glyoxalase II (GLO2, EC 3.1.2.6) is functional in vivo Leishmania major
a

Source Tissue

Source Tissue Comment Organism Textmining
epimastigote
-
 Trypanosoma cruzi
-
additional information in the bloodstream and procyclic form of the recombinant protein of Trpypanosomas brucei, overexpressed with GLO1 of Trypanosomas cruzi Trypanosoma cruzi
-
promastigote
-
 Leishmania major
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.023
-
 recombinant protein, expressed in Trypanosoma brucei which lacks GLO1 activity, procyclic form, pH 7.0, 25°C Trypanosoma cruzi
0.038
-
 recombinant protein, expressed in Trypanosoma brucei which lacks GLO1 activity, bloodstream form, pH 7.0, 25°C Trypanosoma cruzi
0.042
-
 whole cell lysate, pH 7.0, 25°C Trypanosoma cruzi
0.085
-
 whole cell lysate, pH 7.0, 25°C Leishmania major

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
trypanothione + methylglyoxal cell lysate of the recombinant protein, esxpression of GLO1 from Trypanosomas cruzi in Trypanosomas brucei which lacks GLO1 activity, pH 7.0, 25°C Trypanosoma cruzi S,S'-bis((R)-lactoyl)trypanothione the product formation is measured by monitoring at 240 nm ?
trypanothione + methylglyoxal cell lysate, pH 7.0, 25°C Leishmania major S,S'-bis((R)-lactoyl)trypanothione the formation is measured by monitoring at 240 nm ?
trypanothione + methylglyoxal cell lysate, pH 7.0, 25°C Leishmania major Friedlin S,S'-bis((R)-lactoyl)trypanothione the formation is measured by monitoring at 240 nm ?

Synonyms

Synonyms Comment Organism
Glo1
-
 Trypanosoma cruzi
Glo1
-
 Leishmania major
glyoxalase I
-
 Trypanosoma cruzi
glyoxalase I
-
 Leishmania major

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Trypanosoma cruzi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Trypanosoma cruzi