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Literature summary for 4.4.1.5 extracted from

  • Miranda, H.V.; Ferreira, A.E.; Quintas, A.; Cordeiro, C.; Freire, A.P.
    Measuring intracellular enzyme concentrations. Assessing the effect of oxidative stress on the amount of glyoxalase I (2008), Biochem. Mol. Biol. Educ., 36, 135-138.
    View publication on PubMed
Search for more literature for 4.4.1.5

Application

Application Comment Organism
analysis practical experiments for students to determine the effect of exposure to oxidative stress conditions on yeast glyoxalase I, investigation of kinetic parameters Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.03
-
 methylglyoxal the apparent kinetic parameters are determined by varying the hemithioacetal concentration between 0.3 and 1.5 mM, which is obtained by the nonenzymatic reaction between glutathione and methylglyoxal Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
 permeabilized with 0.01% (w/v) digitonin
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information permeabilized cells of Saccharomyces cerevisiae, with 0.01% (w/v) digitonin Saccharomyces cerevisiae
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + 2-oxopropanal
-
 Saccharomyces cerevisiae (R)-S-lactoylglutathione
-
 r
glutathione + methylglyoxal
-
 Saccharomyces cerevisiae S-D-lactoylglutathione
-
 ?
glutathione + methylglyoxal glutathione reacts with methylglyoxal forming a hemithioacetal, and then glyoxlase I catalyses the formation of S-D-lactoylglutathione Saccharomyces cerevisiae S-((R)-lactoyl)glutathione
-
 ?
additional information the glyoxalase I activity is measured spectrophotometrically by S-D-lactoylglutathione formation at 240 nm Saccharomyces cerevisiae ?
-
 ?

Synonyms

Synonyms Comment Organism
glyoxalase I
-
 Saccharomyces cerevisiae
S-D-lactoylglutathione:methylglyoxal lyase
-
 Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
258.3
-
 glutathione the apparent kinetic parameters are determined by using hemithioacetal as substrate, which is obtained by the nonenzymatic reaction between glutathione and methylglyoxal Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 assay at Saccharomyces cerevisiae