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Literature summary for 4.4.1.5 extracted from

  • Greig, N.; Wyllie, S.; Vickers, T.J.; Fairlamb, A.H.
    Trypanothione-dependent glyoxalase I in Trypanosoma cruzi (2006), Biochem. J., 400, 217-223.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
medicine high degree of similarity between the trypanosomatid and bacterial GLO1 proteins, contrasting substrate specificities of human and trypanosomatid glyoxalase enzymes suggest that the glyoxalase system may be an attractive target for anti-trypanosomal chemotherapy Trypanosoma cruzi

Cloned(Commentary)

Cloned (Comment) Organism
pET15b-GLO1 construct expressed in Escherichia coli BL21(DE3)pLysS cells Trypanosoma cruzi

Inhibitors

Inhibitors Comment Organism Structure
additional information S-4-bromobenzylglutathione is inactive as inhibitor Trypanosoma cruzi
S-4-bromobenzylglutathionylspermidine potent linear competitive inhibitor, selectively inhibits trypanosomatid GLO1 activity Trypanosoma cruzi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.008
-
glutathionylspermidine with methylglyoxal Trypanosoma cruzi
0.014
-
glutathionylspermidine with phenylglyoxal Trypanosoma cruzi
0.109
-
trypanothione with methylglyoxal Trypanosoma cruzi
0.207
-
trypanothione with phenylglyoxal Trypanosoma cruzi
1.8
-
glutathione with methylglyoxal Trypanosoma cruzi
1.8
-
glutathione with phenylglyoxal Trypanosoma cruzi

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Trypanosoma cruzi 5829
-
mitochondrion
-
Trypanosoma cruzi 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ only marginally less effective than nickel Trypanosoma cruzi
Mn2+
-
Trypanosoma cruzi
Ni2+ most effective Trypanosoma cruzi
Zn2+
-
Trypanosoma cruzi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
16647
-
x * 17000, SDS-PAGE or Western blot analysis, x * 16647, predicted, x * 16650, MALDI-TOF-MS analysis Trypanosoma cruzi
16650
-
x * 17000, SDS-PAGE or Western blot analysis, x * 16647, predicted, x * 16650, MALDI-TOF-MS analysis Trypanosoma cruzi
17000
-
x * 17000, SDS-PAGE or Western blot analysis, x * 16647, predicted, x * 16650, MALDI-TOF-MS analysis Trypanosoma cruzi
31300
-
size exclusion chromatography and analytical ultracentrifugation Trypanosoma cruzi

Organism

Organism UniProt Comment Textmining
Trypanosoma cruzi
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity via nickel affinity and anion exchange chromatography Trypanosoma cruzi

Source Tissue

Source Tissue Comment Organism Textmining
epimastigote
-
Trypanosoma cruzi
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + methylglyoxal
-
Trypanosoma cruzi S-D-lactoylglutathione
-
?
glutathione + phenylglyoxal
-
Trypanosoma cruzi S-mandoylglutathione
-
?
glutathionylspermidine + methylglyoxal most efficient substrate Trypanosoma cruzi ?
-
?
glutathionylspermidine + phenylglyoxal
-
Trypanosoma cruzi ?
-
?
trypanothione + methylglyoxal
-
Trypanosoma cruzi S,S'-bis((R)-lactoyl)trypanothione
-
?
trypanothione + phenylglyoxal
-
Trypanosoma cruzi S-mandoyltrypanothione
-
?

Subunits

Subunits Comment Organism
dimer x * 17000, SDS-PAGE or Western blot analysis, x * 16647, predicted, x * 16650, MALDI-TOF-MS analysis Trypanosoma cruzi

Synonyms

Synonyms Comment Organism
Glo1
-
Trypanosoma cruzi
glyoxalase I
-
Trypanosoma cruzi

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
105
-
glutathionylspermidine with phenylglyoxal Trypanosoma cruzi
161
-
glutathionylspermidine with methylglyoxal Trypanosoma cruzi
265
-
trypanothione with phenylglyoxal Trypanosoma cruzi
363
-
trypanothione with methylglyoxal Trypanosoma cruzi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Trypanosoma cruzi

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0054
-
S-4-bromobenzylglutathionylspermidine
-
Trypanosoma cruzi