Application | Comment | Organism |
---|---|---|
medicine | high degree of similarity between the trypanosomatid and bacterial GLO1 proteins, contrasting substrate specificities of human and trypanosomatid glyoxalase enzymes suggest that the glyoxalase system may be an attractive target for anti-trypanosomal chemotherapy | Trypanosoma cruzi |
Cloned (Comment) | Organism |
---|---|
pET15b-GLO1 construct expressed in Escherichia coli BL21(DE3)pLysS cells | Trypanosoma cruzi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | S-4-bromobenzylglutathione is inactive as inhibitor | Trypanosoma cruzi | |
S-4-bromobenzylglutathionylspermidine | potent linear competitive inhibitor, selectively inhibits trypanosomatid GLO1 activity | Trypanosoma cruzi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
glutathionylspermidine | with methylglyoxal | Trypanosoma cruzi | |
0.014 | - |
glutathionylspermidine | with phenylglyoxal | Trypanosoma cruzi | |
0.109 | - |
trypanothione | with methylglyoxal | Trypanosoma cruzi | |
0.207 | - |
trypanothione | with phenylglyoxal | Trypanosoma cruzi | |
1.8 | - |
glutathione | with methylglyoxal | Trypanosoma cruzi | |
1.8 | - |
glutathione | with phenylglyoxal | Trypanosoma cruzi |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Trypanosoma cruzi | 5829 | - |
mitochondrion | - |
Trypanosoma cruzi | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | only marginally less effective than nickel | Trypanosoma cruzi | |
Mn2+ | - |
Trypanosoma cruzi | |
Ni2+ | most effective | Trypanosoma cruzi | |
Zn2+ | - |
Trypanosoma cruzi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
16647 | - |
x * 17000, SDS-PAGE or Western blot analysis, x * 16647, predicted, x * 16650, MALDI-TOF-MS analysis | Trypanosoma cruzi |
16650 | - |
x * 17000, SDS-PAGE or Western blot analysis, x * 16647, predicted, x * 16650, MALDI-TOF-MS analysis | Trypanosoma cruzi |
17000 | - |
x * 17000, SDS-PAGE or Western blot analysis, x * 16647, predicted, x * 16650, MALDI-TOF-MS analysis | Trypanosoma cruzi |
31300 | - |
size exclusion chromatography and analytical ultracentrifugation | Trypanosoma cruzi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma cruzi | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity via nickel affinity and anion exchange chromatography | Trypanosoma cruzi |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
epimastigote | - |
Trypanosoma cruzi | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + methylglyoxal | - |
Trypanosoma cruzi | S-D-lactoylglutathione | - |
? | |
glutathione + phenylglyoxal | - |
Trypanosoma cruzi | S-mandoylglutathione | - |
? | |
glutathionylspermidine + methylglyoxal | most efficient substrate | Trypanosoma cruzi | ? | - |
? | |
glutathionylspermidine + phenylglyoxal | - |
Trypanosoma cruzi | ? | - |
? | |
trypanothione + methylglyoxal | - |
Trypanosoma cruzi | S,S'-bis((R)-lactoyl)trypanothione | - |
? | |
trypanothione + phenylglyoxal | - |
Trypanosoma cruzi | S-mandoyltrypanothione | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | x * 17000, SDS-PAGE or Western blot analysis, x * 16647, predicted, x * 16650, MALDI-TOF-MS analysis | Trypanosoma cruzi |
Synonyms | Comment | Organism |
---|---|---|
Glo1 | - |
Trypanosoma cruzi |
glyoxalase I | - |
Trypanosoma cruzi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
105 | - |
glutathionylspermidine | with phenylglyoxal | Trypanosoma cruzi | |
161 | - |
glutathionylspermidine | with methylglyoxal | Trypanosoma cruzi | |
265 | - |
trypanothione | with phenylglyoxal | Trypanosoma cruzi | |
363 | - |
trypanothione | with methylglyoxal | Trypanosoma cruzi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Trypanosoma cruzi |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0054 | - |
S-4-bromobenzylglutathionylspermidine | - |
Trypanosoma cruzi |