Cloned (Comment) | Organism |
---|---|
expression in Saccharomyces cerevisiae | Schizosaccharomyces pombe |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
Phenylglyoxal | pH 7.0, 25°C | Schizosaccharomyces pombe | |
0.8 | - |
glutathione | pH 7.0, 25°C | Schizosaccharomyces pombe | |
1.6 | - |
methylglyoxal | pH 7.0, 25°C | Schizosaccharomyces pombe |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 20mM, 32.8% reactivation of EDTA-treated enzyme | Schizosaccharomyces pombe | |
Co2+ | 1mM, 78.6% reactivation of EDTA-treated enzyme | Schizosaccharomyces pombe | |
Fe2+ | 1mM, 8.4% reactivation of EDTA-treated enzyme | Schizosaccharomyces pombe | |
Mg2+ | 20mM, 13.7% reactivation of EDTA-treated enzyme | Schizosaccharomyces pombe | |
Mn2+ | 1mM, 186% reactivation of EDTA-treated enzyme | Schizosaccharomyces pombe | |
Ni2+ | 1mM, 67.9% reactivation of EDTA-treated enzyme | Schizosaccharomyces pombe | |
Zn2+ | 0.5mM, 84.7% reactivation of EDTA-treated enzyme | Schizosaccharomyces pombe |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
gel filtration | Schizosaccharomyces pombe |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Schizosaccharomyces pombe | - |
PR 109 | - |
Schizosaccharomyces pombe PR 109 | - |
PR 109 | - |
Purification (Comment) | Organism |
---|---|
- |
Schizosaccharomyces pombe |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
Vmax methylglyoxal purified enzyme 2 mmol/min/mg protein, Vmax phenylglyoxal 0.197 mmol/min/mg protein, pH 7.0, 25°C | Schizosaccharomyces pombe |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + methylglyoxal | - |
Schizosaccharomyces pombe | (R)-S-lactoylglutathione | - |
? | |
glutathione + methylglyoxal | - |
Schizosaccharomyces pombe PR 109 | (R)-S-lactoylglutathione | - |
? | |
glutathione + phenylglyoxal | - |
Schizosaccharomyces pombe | S-mandeloylglutathione | - |
? | |
glutathione + phenylglyoxal | - |
Schizosaccharomyces pombe PR 109 | S-mandeloylglutathione | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 34000, SDS-PAGE | Schizosaccharomyces pombe |
Synonyms | Comment | Organism |
---|---|---|
Glo1 | - |
Schizosaccharomyces pombe |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
100% activity after 4 h incubation at 60°C, 50% inactivation after 150 min incubation at 64°C or after 60 min incubation at 65°C | Schizosaccharomyces pombe |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 10 | stable at 4°C for at least 24h | Schizosaccharomyces pombe |