Literature summary for 4.4.1.5 extracted from

Frickel, E.M.; Jemth, P.; Widersten, M.; Mannervik, B.
Yeast glyoxalase I is a monomeric enzyme with two active sites (2001), J. Biol. Chem., 276, 1845-1849.

Search for more literature for 4.4.1.5

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Saccharomyces cerevisiae

Protein Variants

Protein Variants	Comment	Organism
E163Q	58% of wild-type kcat	Saccharomyces cerevisiae
E163Q/E318Q	0.02% of wild-type kcat	Saccharomyces cerevisiae
E318Q	16% of wild-type kcat	Saccharomyces cerevisiae

Inhibitors

Inhibitors	Comment	Organism	Structure
S-hexylglutathione	-	Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.41	-	glutathione-methylglyoxal hemithioacetal	30°C, pH 7.0	Saccharomyces cerevisiae

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	at least one iron per enzyme molecule, the second metal is zinc or manganese, apparantly depending on growth conditions	Saccharomyces cerevisiae
Mn2+	can replace Zn2+	Saccharomyces cerevisiae
Zn2+	-	Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
glutathione-methylglyoxal hemithioacetal	Saccharomyces cerevisiae	glutathione-methylglyoxal hemithioacetal is formed non-enzymatically from methylglyoxal and glutathione	(R)-S-lactoylglutathione	-	?

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant glyoxalase I	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
glutathione-methylglyoxal hemithioacetal	glutathione-methylglyoxal hemithioacetal is formed non-enzymatically from methylglyoxal and glutathione	Saccharomyces cerevisiae	(R)-S-lactoylglutathione	-	?

Subunits

Subunits	Comment	Organism
monomer	single polypeptide with two active sites that catalyze the same reaction	Saccharomyces cerevisiae

Synonyms

Synonyms	Comment	Organism
glyoxalase I	-	Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1700	-	glutathione-methylglyoxal hemithioacetal	30°C, pH 7.0	Saccharomyces cerevisiae

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.015	-	S-hexylglutathione	-	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)