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Literature summary for 4.4.1.5 extracted from
- A 33-kDa allergen from rice (Oryza sativa L. Japonica). cDNA cloning, expression, and identification as a novel glyoxalase I (2001), J. Biol. Chem., 276, 11376-11381.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloning of cDNA, expression in Escherichia coli | Oryza sativa Japonica Group |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 7.6 | - |
glutathione-methylglyoxal hemithioacetal | 25°C, pH 7.0, native Glb33 | Oryza sativa Japonica Group |  |
| 11 | - |
glutathione-methylglyoxal hemithioacetal | 25°C, pH 7.0, recombinant Glb33 | Oryza sativa Japonica Group | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
x * 33000, SDS-PAGE, native and recombinant Glb33 | Oryza sativa Japonica Group |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione-methylglyoxal hemithioacetal | Oryza sativa Japonica Group | glutathione-methylglyoxal hemithioacetal is formed non-enzymatically from methylglyoxal and glutathione | (R)-S-lactoylglutathione | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryza sativa Japonica Group | Q948T6 | L. Japonica | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native and recombinant glyoxalase I | Oryza sativa Japonica Group |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione-methylglyoxal hemithioacetal | glutathione-methylglyoxal hemithioacetal is formed non-enzymatically from methylglyoxal and glutathione | Oryza sativa Japonica Group | (R)-S-lactoylglutathione | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 33000, SDS-PAGE, native and recombinant Glb33 | Oryza sativa Japonica Group |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Glb33 | - |
Oryza sativa Japonica Group |
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Release 2023.1 (January 2023)
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