Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
H5Q | active in the presence of both Ni2+ and Zn2+ | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0089 | - |
glutathione-methylglyoxal hemithioacetal | in the presence of Cd2+ | Escherichia coli | |
0.01 | - |
glutathione-methylglyoxal hemithioacetal | in the presence of Mn2+ | Escherichia coli | |
0.01 | - |
glutathione-methylglyoxal hemithioacetal | in the presence of Fe2+ | Escherichia coli | |
0.012 | - |
glutathione-methylglyoxal hemithioacetal | in the presence of Co+ | Escherichia coli | |
0.027 | - |
glutathione-methylglyoxal hemithioacetal | in the presence of Ni2+ | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | 6% of activity with Ni2+ | Escherichia coli | |
Co2+ | 31% of activity with Ni2+ | Escherichia coli | |
Fe2+ | 16% of activity with Ni2+ | Escherichia coli | |
Mn2+ | 18% of activity with Ni2+ | Escherichia coli | |
additional information | no activity with Zn2+ | Escherichia coli | |
Ni2+ | required for activity | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione-methylglyoxal hemithioacetal | Escherichia coli | glutathione-methylglyoxal hemithioacetal is formed non-enzymatically from methylglyoxal and glutathione | (R)-S-lactoylglutathione | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione-methylglyoxal hemithioacetal | glutathione-methylglyoxal hemithioacetal is formed non-enzymatically from methylglyoxal and glutathione | Escherichia coli | (R)-S-lactoylglutathione | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GLXI | - |
Escherichia coli |
glyoxalase I | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.5 | - |
glutathione-methylglyoxal hemithioacetal | in the presence of Fe2+ | Escherichia coli | |
8 | - |
glutathione-methylglyoxal hemithioacetal | in the presence of Mn2+ | Escherichia coli | |
21.4 | - |
glutathione-methylglyoxal hemithioacetal | in the presence of Cd2+ | Escherichia coli | |
55.7 | - |
glutathione-methylglyoxal hemithioacetal | in the presence of Fe2+ | Escherichia coli | |
60.2 | - |
glutathione-methylglyoxal hemithioacetal | in the presence of Mn2+ | Escherichia coli | |
106 | - |
glutathione-methylglyoxal hemithioacetal | in the presence of Co+ | Escherichia coli | |
338 | - |
glutathione-methylglyoxal hemithioacetal | in the presence of Ni2+ | Escherichia coli |