Literature summary for 4.4.1.5 extracted from

Creighton, D.J.; Hamilton, D.S.
Brief history of glyoxalase I and what we have learned about metal ion-dependent, enzyme-catalyzed isomerizations (2001), Arch. Biochem. Biophys., 387, 1-10.

Search for more literature for 4.4.1.5

Crystallization (Commentary)

Crystallization (Comment)	Organism
-	Escherichia coli
crystal structure of glyoxalase I complexed with S-benzylglutathione and S-(N-p-iodophenyl-N-hydroxycarbamoyl)glutathione	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
E172Q	100000fold lower activity than wild-type	Homo sapiens
E99Q	10000fold lower activity than wild-type	Homo sapiens
E99Q/Q33E	100fold lower activity than wild-type	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
S-(N-p-iodophenyl-N-hydroxycarbamoyl)glutathione	-	Homo sapiens
S-benzylglutathione	-	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	66% of activity with Zn2+	Homo sapiens
Mg2+	101% of activity with Zn2+	Homo sapiens
Mn2+	67% of activity with Zn2+	Homo sapiens
Zn2+	required for activity	Homo sapiens
Zn2+	required for activity, 1 Zn2+ per subunit	Saccharomyces cerevisiae
Zn2+	required for activity, no activity with Ni2+, Co2+ and Cd2+	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
glutathione-methylglyoxal hemithioacetal	Escherichia coli	glutathione-methylglyoxal hemithioacetal is formed non-enzymatically from methylglyoxal and glutathione	(R)-S-lactoylglutathione	-	?
glutathione-methylglyoxal hemithioacetal	Saccharomyces cerevisiae	glutathione-methylglyoxal hemithioacetal is formed non-enzymatically from methylglyoxal and glutathione	(R)-S-lactoylglutathione	-	?
glutathione-methylglyoxal hemithioacetal	Homo sapiens	glutathione-methylglyoxal hemithioacetal is formed non-enzymatically from methylglyoxal and glutathione, operates in conjunction with glyoxalase II to convert cytotoxic methylglyoxal to nontoxic D-lactate	(R)-S-lactoylglutathione	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-
Homo sapiens	-	-	-
Saccharomyces cerevisiae	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
glutathione-methylglyoxal hemithioacetal	glutathione-methylglyoxal hemithioacetal is formed non-enzymatically from methylglyoxal and glutathione	Escherichia coli	(R)-S-lactoylglutathione	-	?
glutathione-methylglyoxal hemithioacetal	glutathione-methylglyoxal hemithioacetal is formed non-enzymatically from methylglyoxal and glutathione	Homo sapiens	(R)-S-lactoylglutathione	-	?
glutathione-methylglyoxal hemithioacetal	glutathione-methylglyoxal hemithioacetal is formed non-enzymatically from methylglyoxal and glutathione	Saccharomyces cerevisiae	(R)-S-lactoylglutathione	-	?
glutathione-methylglyoxal hemithioacetal	glutathione-methylglyoxal hemithioacetal is formed non-enzymatically from methylglyoxal and glutathione, operates in conjunction with glyoxalase II to convert cytotoxic methylglyoxal to nontoxic D-lactate	Homo sapiens	(R)-S-lactoylglutathione	-	?

Synonyms

Synonyms	Comment	Organism
GLXI	-	Homo sapiens
glyoxalase I	-	Escherichia coli
glyoxalase I	-	Homo sapiens
glyoxalase I	-	Saccharomyces cerevisiae

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.00001	-	S-(N-p-iodophenyl-N-hydroxycarbamoyl)glutathione	-	Homo sapiens
0.00028	-	S-benzylglutathione	-	Homo sapiens

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Release 2023.1 (January 2023)