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Literature summary for 4.4.1.5 extracted from
- Purification and properties of glyoxalase I from sheep liver (1975), Eur. J. Biochem., 52, 493-503.
General Stability
| General Stability | Organism |
|---|---|
| native enzyme is fully stable in dialysis in contrast to the metal-free enzyme | Ovis aries |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Ovis aries |  |
| 2,2'-dipyridyl | - |
Ovis aries | |
| Chelex-100 | - |
Ovis aries | |
| EDTA | - |
Ovis aries | |
| EGTA | - |
Ovis aries | |
| formaldehyde | - |
Ovis aries | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.078 | - |
methylglyoxal | - |
Ovis aries | |
| 0.113 | - |
Phenylglyoxal | - |
Ovis aries | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates apoenzyme | Ovis aries | |
| Co2+ | reactivates apoenzyme | Ovis aries | |
| Mg2+ | activates apoenzyme | Ovis aries | |
| Mn2+ | activates apoenzyme | Ovis aries | |
| Ni2+ | activates apoenzyme | Ovis aries | |
| Zn2+ | reactivates apoenzyme | Ovis aries | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45900 | - |
gel filtration | Ovis aries |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ovis aries | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Ovis aries | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Ovis aries |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C or -70°C, native enzyme loses 0-10% of activity, metal-free enzyme loses 30-40% of activity during 40 days | Ovis aries |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione + glyoxal | - |
Ovis aries | S-glycolylglutathione | - |
? | |
| glutathione + hydroxypyruvaldehyde | - |
Ovis aries | ? | - |
? | |
| glutathione + kethoxal | - |
Ovis aries | ? | - |
? | |
| glutathione + methylglyoxal | - |
Ovis aries | S-lactoylglutathione | - |
? | |
| glutathione + phenylglyoxal | - |
Ovis aries | S-mandeloylglutathione | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 7.5 | - |
Ovis aries |
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Release 2023.1 (January 2023)
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