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Literature summary for 4.4.1.36 extracted from

  • Hu, W.; Song, H.; Sae Her, A.; Bak, D.W.; Naowarojna, N.; Elliott, S.J.; Qin, L.; Chen, X.; Liu, P.
    Bioinformatic and biochemical characterizations of C-S bond formation and cleavage enzymes in the fungus Neurospora crassa ergothioneine biosynthetic pathway (2014), Org. Lett., 16, 5382-5385 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.4.1.36

Application

Application Comment Organism
synthesis the ergothioneine biosynthetic pathway (EgtA-EgtE catalysis) provides an opportunity for ergothioneine production through metabolic engineering Neurospora crassa

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Neurospora crassa
gene NCU11365, genome mining, cloning from a sequence determined by screening of an EgtB homologue sequence similarity network from Mycobacterum smegmatis EgtB, recombinant expression of enzyme EgtE in Escherichia coli strain BL21(DE3) Neurospora crassa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1947
-
 S-(hercyn-2-yl)-L-cysteine S-oxide pH and temperature not specified in the publication Neurospora crassa
0.195
-
 S-(hercyn-2-yl)-L-cysteine S-oxide pH not specified in the publication, temperature not specified in the publication Neurospora crassa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-(hydroxysulfanyl)hercynine + reduced acceptor Neurospora crassa
-
 ergothioneine + acceptor + H2O
-
 ?
2-(hydroxysulfanyl)hercynine + reduced acceptor Neurospora crassa DSM 1257
-
 ergothioneine + acceptor + H2O
-
 ?
2-(hydroxysulfanyl)hercynine + reduced acceptor Neurospora crassa ATCC 24698
-
 ergothioneine + acceptor + H2O
-
 ?
S-(hercyn-2-yl)-L-cysteine S-oxide + H2O Neurospora crassa
-
 2-(hydroxysulfanyl)hercynine + pyruvate + ammonia
-
 ?
S-(hercyn-2-yl)-L-cysteine S-oxide + H2O Neurospora crassa DSM 1257
-
 2-(hydroxysulfanyl)hercynine + pyruvate + ammonia
-
 ?
S-(hercyn-2-yl)-L-cysteine S-oxide + H2O Neurospora crassa ATCC 24698
-
 2-(hydroxysulfanyl)hercynine + pyruvate + ammonia
-
 ?
S-(hercyn-2-yl)-L-cysteine S-oxide + reduced acceptor Neurospora crassa
-
 ergothioneine + pyruvate + ammonia + acceptor
-
 ?
S-(hercyn-2-yl)-L-cysteine S-oxide + reduced acceptor Neurospora crassa overall reaction ergothioneine + pyruvate + ammonia + acceptor
-
 ?
S-(hercyn-2-yl)-L-cysteine S-oxide + reduced acceptor Neurospora crassa DSM 1257
-
 ergothioneine + pyruvate + ammonia + acceptor
-
 ?
S-(hercyn-2-yl)-L-cysteine S-oxide + reduced acceptor Neurospora crassa DSM 1257 overall reaction ergothioneine + pyruvate + ammonia + acceptor
-
 ?
S-(hercyn-2-yl)-L-cysteine S-oxide + reduced acceptor Neurospora crassa ATCC 24698
-
 ergothioneine + pyruvate + ammonia + acceptor
-
 ?
S-(hercyn-2-yl)-L-cysteine S-oxide + reduced acceptor Neurospora crassa ATCC 24698 overall reaction ergothioneine + pyruvate + ammonia + acceptor
-
 ?

Organism

Organism UniProt Comment Textmining
Neurospora crassa A7UX13
-
-
Neurospora crassa ATCC 24698 A7UX13
-
-
Neurospora crassa DSM 1257 A7UX13
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli strain BL21(DE3) Neurospora crassa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-(hydroxysulfanyl)hercynine + reduced acceptor
-
 Neurospora crassa ergothioneine + acceptor + H2O
-
 ?
2-(hydroxysulfanyl)hercynine + reduced acceptor
-
 Neurospora crassa DSM 1257 ergothioneine + acceptor + H2O
-
 ?
2-(hydroxysulfanyl)hercynine + reduced acceptor
-
 Neurospora crassa ATCC 24698 ergothioneine + acceptor + H2O
-
 ?
additional information enzyme activity determination by a 1H NMR assay of chemical shift of the imidazole hydrogen atoms and a colorimetric assay by coupling the C-S lyase reaction with lactate dehydrogenas, product determination by high-resolution mass spectrometry and NMR Neurospora crassa ?
-
 ?
additional information enzyme activity determination by a 1H NMR assay of chemical shift of the imidazole hydrogen atoms and a colorimetric assay by coupling the C-S lyase reaction with lactate dehydrogenas, product determination by high-resolution mass spectrometry and NMR Neurospora crassa DSM 1257 ?
-
 ?
additional information enzyme activity determination by a 1H NMR assay of chemical shift of the imidazole hydrogen atoms and a colorimetric assay by coupling the C-S lyase reaction with lactate dehydrogenas, product determination by high-resolution mass spectrometry and NMR Neurospora crassa ATCC 24698 ?
-
 ?
S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
 Neurospora crassa 2-(hydroxysulfanyl)hercynine + pyruvate + ammonia
-
 ?
S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
 Neurospora crassa DSM 1257 2-(hydroxysulfanyl)hercynine + pyruvate + ammonia
-
 ?
S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
 Neurospora crassa ATCC 24698 2-(hydroxysulfanyl)hercynine + pyruvate + ammonia
-
 ?
S-(hercyn-2-yl)-L-cysteine S-oxide + reduced acceptor
-
 Neurospora crassa ergothioneine + pyruvate + ammonia + acceptor
-
 ?
S-(hercyn-2-yl)-L-cysteine S-oxide + reduced acceptor overall reaction Neurospora crassa ergothioneine + pyruvate + ammonia + acceptor
-
 ?
S-(hercyn-2-yl)-L-cysteine S-oxide + reduced acceptor
-
 Neurospora crassa DSM 1257 ergothioneine + pyruvate + ammonia + acceptor
-
 ?
S-(hercyn-2-yl)-L-cysteine S-oxide + reduced acceptor overall reaction Neurospora crassa DSM 1257 ergothioneine + pyruvate + ammonia + acceptor
-
 ?
S-(hercyn-2-yl)-L-cysteine S-oxide + reduced acceptor
-
 Neurospora crassa ATCC 24698 ergothioneine + pyruvate + ammonia + acceptor
-
 ?
S-(hercyn-2-yl)-L-cysteine S-oxide + reduced acceptor overall reaction Neurospora crassa ATCC 24698 ergothioneine + pyruvate + ammonia + acceptor
-
 ?

Synonyms

Synonyms Comment Organism
Egt2
-
 Neurospora crassa
egtE
-
 Neurospora crassa
hercynylcysteine sulfoxide lyase
-
 Neurospora crassa
hercynylcysteine sulfoxide lyase UniProt Neurospora crassa
NCU01256 protein
-
 Neurospora crassa
NCU11365
-
 Neurospora crassa
PLP-dependent C-S lyase
-
 Neurospora crassa

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Neurospora crassa

General Information

General Information Comment Organism
metabolism the enzyme catalyzes a pyridoxal 5'-phosphate-mediated C-S lyase reaction in the ergothioneine biosynthesis Neurospora crassa