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Literature summary for 4.4.1.35 extracted from

  • Ukai, K.; Sekiya, J.
    Rapid purification and characterization of cystine lyase b from broccoli inflorescence (1999), Phytochemistry, 51, 853-859.
No PubMed abstract available
Search for more literature for 4.4.1.35

Inhibitors

Inhibitors Comment Organism Structure
carboxymethoxylamine 0.5 mM, 2% residual activity; 0.5 mM, complete inhibition Brassica oleracea var. italica
hydroxylamine 0.5 mM, 15% residual activity; 0.5 mM, complete inhibition Brassica oleracea var. italica
additional information not inhibitory at 0.5 mM, iodoacetate, N-ethylmaleimide, EDTA; not inhibitory: iodoacetate, N-ethylmaleimide and EDTA Brassica oleracea var. italica

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol and/or vacuole Brassica oleracea var. italica 5829
-
vacuole and/or cytosol Brassica oleracea var. italica 5773
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 4 * 40000, SDS-PAGE Brassica oleracea var. italica
45000
-
 4 * 45000, SDS-PAGE Brassica oleracea var. italica
160000
-
 gel filtration Brassica oleracea var. italica
186000
-
 gel filtration Brassica oleracea var. italica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-aminoprop-2-enoate Brassica oleracea var. italica
-
 2-iminopropanoate spontaneous partial reaction ?
2-iminopropanoate + H2O Brassica oleracea var. italica
-
 pyruvate + NH3 spontaneous partial reaction ?
L-cystine Brassica oleracea var. italica
-
 L-thiocysteine + 2-aminoprop-2-enoate
-
 ?
L-cystine + H2O Brassica oleracea var. italica
-
 L-thiocysteine + pyruvate + NH3 overall reaction ?
additional information Brassica oleracea var. italica the enzyme catalyzes beta-elimination of L-cystine to yield thiocysteine, pyruvate and possibly ammonia. S-Methyl L-cysteine sulfoxide and S-ethyl L-cysteine sulfoxide are substrates but are less suitable than L-cystine. No substrates: D-cystine, L- and o-cysteine and cystathionine ?
-
 ?
S-ethyl L-cysteine sulfoxide + H2O Brassica oleracea var. italica 48% of the activity with L-cystine ?
-
 ?
S-methyl L-cysteine sulfoxide + H2O Brassica oleracea var. italica 37% of the activity with L-cystine ?
-
 ?

Organism

Organism UniProt Comment Textmining
Brassica oleracea var. italica
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
no glycoprotein
-
 Brassica oleracea var. italica

Purification (Commentary)

Purification (Comment) Organism
-
 Brassica oleracea var. italica
isoform cystine lyase b, rapid purifiaction method Brassica oleracea var. italica

Source Tissue

Source Tissue Comment Organism Textmining
inflorescence
-
 Brassica oleracea var. italica
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
29.8
-
 pH 8.0, 30°C Brassica oleracea var. italica

Storage Stability

Storage Stability Organism
-20°C, little loss of activity during 6 months Brassica oleracea var. italica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-aminoprop-2-enoate
-
 Brassica oleracea var. italica 2-iminopropanoate spontaneous partial reaction ?
2-iminopropanoate + H2O
-
 Brassica oleracea var. italica pyruvate + NH3 spontaneous partial reaction ?
L-cystine
-
 Brassica oleracea var. italica L-thiocysteine + 2-aminoprop-2-enoate
-
 ?
L-cystine + H2O
-
 Brassica oleracea var. italica L-thiocysteine + pyruvate + NH3
-
 ?
L-cystine + H2O
-
 Brassica oleracea var. italica L-thiocysteine + pyruvate + NH3 overall reaction ?
additional information the enzyme catalyzes beta-elimination of L-cystine to yield thiocysteine, pyruvate and possibly ammonia. S-Methyl L-cysteine sulfoxide and S-ethyl L-cysteine sulfoxide are substrates but are less suitable than L-cystine. No substrates: D-cystine, L- and o-cysteine and cystathionine Brassica oleracea var. italica ?
-
 ?
S-ethyl L-cysteine sulfoxide + H2O
-
 Brassica oleracea var. italica ?
-
 ?
S-ethyl L-cysteine sulfoxide + H2O 48% of the activity with L-cystine Brassica oleracea var. italica ?
-
 ?
S-ethyl L-cysteine sulfoxide + H2O 64% of the activity with L-cystine Brassica oleracea var. italica ?
-
 ?
S-methyl L-cysteine sulfoxide + H2O
-
 Brassica oleracea var. italica ?
-
 ?
S-methyl L-cysteine sulfoxide + H2O 37% of the activity with L-cystine Brassica oleracea var. italica ?
-
 ?
S-methyl L-cysteine sulfoxide + H2O 46% of the activity with L-cystine Brassica oleracea var. italica ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 45000, SDS-PAGE Brassica oleracea var. italica
tetramer 4 * 40000, SDS-PAGE Brassica oleracea var. italica

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 40
-
 Brassica oleracea var. italica

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 5 min, stable Brassica oleracea var. italica
40
-
 10 min, no loss of activity Brassica oleracea var. italica
50
-
 5 min, 70% loss of activity Brassica oleracea var. italica
50
-
 10 min, 60% loss of activity Brassica oleracea var. italica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Brassica oleracea var. italica

pH Range

pH Minimum pH Maximum Comment Organism
3 11 more than 50% of maximum activity Brassica oleracea var. italica

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Brassica oleracea var. italica