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Literature summary for 4.4.1.28 extracted from
- Characterization of L-cysteine desulfhydrase from Prevotella intermedia (2009), Oral Microbiol. Immunol., 24, 485-492.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Prevotella intermedia |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D198N | mutation resuklts in complete disappearance of the L-cysteine desulfhydrase activity | Prevotella intermedia |
| K233N | mutation resuklts in complete disappearance of the L-cysteine desulfhydrase activity | Prevotella intermedia |
| R365G | mutant enzyme retains catalytic activity as well as the wild-type enzyme | Prevotella intermedia |
| Y118A | mutation resuklts in complete disappearance of the L-cysteine desulfhydrase activity | Prevotella intermedia |
| Y59A | mutation resuklts in complete disappearance of the L-cysteine desulfhydrase activity | Prevotella intermedia |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.7 | - |
L-cysteine | pH 7.0, 37°C | Prevotella intermedia |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| ZnCl2 | 1 mM, completely inhibits | Prevotella intermedia | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44000 | - |
x * 44000, SDS-PAGE | Prevotella intermedia |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Prevotella intermedia | B9X1L2 | - |
- |
| Prevotella intermedia ATCC 25611 | B9X1L2 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Prevotella intermedia |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.2 | - |
pH 7.0, 37°C | Prevotella intermedia |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-cysteine + H2O | DL-homocysteine, cysteinyl glycine, and glutathione are not substrates to produce hydrogen sulfide | Prevotella intermedia | sulfide + NH3 + pyruvate | - |
? | |
| L-cysteine + H2O | DL-homocysteine, cysteinyl glycine, and glutathione are not substrates to produce hydrogen sulfide | Prevotella intermedia ATCC 25611 | sulfide + NH3 + pyruvate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 44000, SDS-PAGE | Prevotella intermedia |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| les | - |
Prevotella intermedia |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Prevotella intermedia |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | required | Prevotella intermedia | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the enzyme is closely associated with the pathogenesis of Prevotella intermedia | Prevotella intermedia |
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Release 2023.1 (January 2023)
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