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Literature summary for 4.4.1.21 extracted from

  • Wang, Y.; Yi, L.; Wang, S.; Fan, H.; Ding, C.; Mao, X.; Lu, C.
    Crystal structure and identification of two key amino acids involved in AI-2 production and biofilm formation in Streptococcus suis LuxS (2015), PLoS ONE, 10, e0138826 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.4.1.21

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Streptococcus suis

Crystallization (Commentary)

Crystallization (Comment) Organism
to 1.93 A resolution Streptococcus suis

Protein Variants

Protein Variants Comment Organism
C82A inactive, mutation decreases autoinducer AI-2 production and biofilm formation Streptococcus suis
C82S 43fold decrease in activity, mutation decreases autoinducer AI-2 production and biofilm formation Streptococcus suis
F80M 7fold decrease in activity, mutation decreases autoinducer AI-2 production and biofilm formation Streptococcus suis
F80M/H87Y 42fold decrease in activity, mutation decreases autoinducer AI-2 production and biofilm formation Streptococcus suis
H87Y 37fold decrease in activity, mutation decreases autoinducer AI-2 production and biofilm formation Streptococcus suis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0164
-
 S-ribosylhomocysteine wild-type, pH 7.5, 37°C Streptococcus suis
0.0223
-
 S-ribosylhomocysteine mutant F80M, pH 7.5, 37°C Streptococcus suis
0.0577
-
 S-ribosylhomocysteine mutant C82S, pH 7.5, 37°C Streptococcus suis
0.0604
-
 S-ribosylhomocysteine mutant F80M/H87Y, pH 7.5, 37°C Streptococcus suis
0.0708
-
 S-ribosylhomocysteine mutant H87Y, pH 7.5, 37°C Streptococcus suis

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ calculated molar ratio of Zn2+ ion to each LuxS monomer is about 1:1 Streptococcus suis

Organism

Organism UniProt Comment Textmining
Streptococcus suis B2CMA5
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Streptococcus suis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-ribosylhomocysteine
-
 Streptococcus suis L-homocysteine + 4,5-dihydroxy-2,3-pentanedione
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.009
-
 S-ribosylhomocysteine mutant F80M/H87Y, pH 7.5, 37°C Streptococcus suis
0.01
-
 S-ribosylhomocysteine mutant C82S, pH 7.5, 37°C Streptococcus suis
0.011
-
 S-ribosylhomocysteine mutant H87Y, pH 7.5, 37°C Streptococcus suis
0.02
-
 S-ribosylhomocysteine mutant F80M, pH 7.5, 37°C Streptococcus suis
0.102
-
 S-ribosylhomocysteine wild-type, pH 7.5, 37°C Streptococcus suis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.149
-
 S-ribosylhomocysteine mutant F80M/H87Y, pH 7.5, 37°C Streptococcus suis
0.153
-
 S-ribosylhomocysteine mutant H87Y, pH 7.5, 37°C Streptococcus suis
0.168
-
 S-ribosylhomocysteine mutant C82S, pH 7.5, 37°C Streptococcus suis
0.882
-
 S-ribosylhomocysteine mutant F80M, pH 7.5, 37°C Streptococcus suis
6.21
-
 S-ribosylhomocysteine wild-type, pH 7.5, 37°C Streptococcus suis