Literature summary for 4.4.1.20 extracted from

Ago, H.; Kanaoka, Y.; Irikura, D.; Lam, B.K.; Shimamura, T.; Austen, K.F.; Miyano, M.
Crystal structure of a human membrane protein involved in cysteinyl leukotriene biosynthesis (2007), Nature, 448, 609-612.

Search for more literature for 4.4.1.20

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression in fission yeast with a His6 tag at the C terminus	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
LTC4S crystallized with glutathione, The structure is determined by the multiwavelength anomalous diffraction method by using the diffraction images from native LTC4S and a selenomethionine-substituted Leu121Met mutant	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
L121M	by site-directed mutagenesis to prepare the selenomethionine-substituted LTC4S, the mutant yeast clone is grown with selenomethionine, purified and crystallized similarly to the wild-type enzyme	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
18000	-	-	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q16873	-	-

Purification (Commentary)

Purification (Comment)	Organism
solubilized with sodium deoxycholic acid and DDM, four steps of column chromatography, including affinity chromatography with S-hexyl GSH	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
leukotriene A4 + glutathione	-	Homo sapiens	leukotriene C4	-	?

Subunits

Subunits	Comment	Organism
trimer	-	Homo sapiens

Synonyms

Synonyms	Comment	Organism
leukotriene C4 synthetase	-	Homo sapiens
LTC4 synthase	-	Homo sapiens
LTC4S	-	Homo sapiens

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Release 2023.1 (January 2023)