BRENDA - Enzyme Database
show all sequences of 4.4.1.17

Import of cytochrome c into mitochondria. Cytochrome c heme lyase

Nicholson, D.W.; Köhler, H.; Neupert, W.; Eur. J. Biochem. 164, 147-157 (1987)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
Cytosolic factor
requirement
Neurospora crassa
glutathione
activation
Neurospora crassa
additional information
independent of a potential across the inner mitochondrial membrane generated by N,N,N',N'-tetramethyl-p-phenylenediamine/ascorbate
Neurospora crassa
NADH
10fold activation between 1 and 10 mM of NADH
Neurospora crassa
NADPH
about half of the activation observed with NADH
Neurospora crassa
Inhibitors
Inhibitors
Commentary
Organism
Structure
Deuterohemin
r; reversible inhibition
Neurospora crassa
hemin
at concentrations higher than 0.002 mM; at higher concentrations
Neurospora crassa
N-ethylmaleimide
kinetics, in vivo; pretreatment of reticulocyte lysates with N-ethylmaleimide results in nearly complete inactivation
Neurospora crassa
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrial inner membrane
-
Neurospora crassa
5743
-
mitochondrion
intermembrane space
Neurospora crassa
5739
-
soluble
-
Neurospora crassa
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Neurospora crassa
-
wild-type strain 74A
-
Purification (Commentary)
Commentary
Organism
partial; partial, subfractionation of mitochondria with digitonin
Neurospora crassa
Source Tissue
Source Tissue
Commentary
Organism
Textmining
hypha
-
Neurospora crassa
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
activity is independent of a potential across the inner mitochondrial membrane generated by N,N,N',N'-tetramethyl-p-phenylenediamine/ascorbate, requires an unidentified cytosolic factor
Neurospora crassa
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Apocytochrome c + heme
catalyzes covalent attachment of heme group to two cysteine residues in the protein
94057
Neurospora crassa
Holocytochrome c
-
94057
Neurospora crassa
?
additional information
distinct from apocytochrome c binding protein
94057
Neurospora crassa
?
-
94057
Neurospora crassa
-
additional information
distinct from apocytochrome c binding protein
94057
Neurospora crassa
?
-
94057
Neurospora crassa
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
-
Neurospora crassa
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Neurospora crassa
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5.5
8.5
about half-maximal activity at pH 5.5 and 8.5
Neurospora crassa
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
activation
Neurospora crassa
NADPH
activation
Neurospora crassa
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
Cytosolic factor
requirement
Neurospora crassa
glutathione
activation
Neurospora crassa
additional information
independent of a potential across the inner mitochondrial membrane generated by N,N,N',N'-tetramethyl-p-phenylenediamine/ascorbate
Neurospora crassa
NADH
10fold activation between 1 and 10 mM of NADH
Neurospora crassa
NADPH
about half of the activation observed with NADH
Neurospora crassa
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
activation
Neurospora crassa
NADPH
activation
Neurospora crassa
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Deuterohemin
r; reversible inhibition
Neurospora crassa
hemin
at concentrations higher than 0.002 mM; at higher concentrations
Neurospora crassa
N-ethylmaleimide
kinetics, in vivo; pretreatment of reticulocyte lysates with N-ethylmaleimide results in nearly complete inactivation
Neurospora crassa
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrial inner membrane
-
Neurospora crassa
5743
-
mitochondrion
intermembrane space
Neurospora crassa
5739
-
soluble
-
Neurospora crassa
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
partial; partial, subfractionation of mitochondria with digitonin
Neurospora crassa
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
hypha
-
Neurospora crassa
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
activity is independent of a potential across the inner mitochondrial membrane generated by N,N,N',N'-tetramethyl-p-phenylenediamine/ascorbate, requires an unidentified cytosolic factor
Neurospora crassa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Apocytochrome c + heme
catalyzes covalent attachment of heme group to two cysteine residues in the protein
94057
Neurospora crassa
Holocytochrome c
-
94057
Neurospora crassa
?
additional information
distinct from apocytochrome c binding protein
94057
Neurospora crassa
?
-
94057
Neurospora crassa
-
additional information
distinct from apocytochrome c binding protein
94057
Neurospora crassa
?
-
94057
Neurospora crassa
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
-
Neurospora crassa
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Neurospora crassa
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5.5
8.5
about half-maximal activity at pH 5.5 and 8.5
Neurospora crassa
Other publictions for EC 4.4.1.17
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
747125
Babbitt
Biosynthesis of single thioet ...
Homo sapiens
Biochemistry
56
3337-3346
2017
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749183
Mendez
Engineered holocytochrome c s ...
Homo sapiens
Proc. Natl. Acad. Sci. USA
114
2235-2240
2017
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1
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2
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4
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748211
Babbitt
Molecular basis behind inabil ...
Homo sapiens
J. Biol. Chem.
291
17523-17534
2016
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1
1
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729264
Babbitt
Conserved residues of the huma ...
Homo sapiens
Biochemistry
53
5261-5271
2014
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1
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12
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2
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1
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3
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1
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1
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12
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1
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2
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2
2
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729740
Zhang
Substrate recognition of holoc ...
Saccharomyces cerevisiae
FEBS Lett.
588
3367-3374
2014
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1
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11
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1
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3
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11
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3
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730058
Babbitt
Mechanisms of mitochondrial ho ...
Homo sapiens
J. Biol. Chem.
289
28795-18807
2014
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730827
San Francisco
Human mitochondrial holocytoch ...
Homo sapiens
Proc. Natl. Acad. Sci. USA
110
E788-E797
2013
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1
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1
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3
1
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1
1
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715034
Stevens
The mitochondrial cytochrome c ...
Saccharomyces cerevisiae
FEBS Lett.
585
1891-1896
2011
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729727
Moore
Mitochondrial cytochrome c syn ...
Saccharomyces cerevisiae
FEBS Lett.
585
3415-3419
2011
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1
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9
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3
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1
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7
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9
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1
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1
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730322
Kleingardner
Comparing substrate specificit ...
Saccharomyces cerevisiae
Metallomics
3
396-403
2011
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1
1
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1
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1
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6
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3
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2
2
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713714
Qidwai
Deletions of Xp provide eviden ...
Homo sapiens
Am. J. Med. Genet. A
152
1588-1590
2010
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1
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1
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715120
Corvest
c-type cytochrome assembly in ...
Saccharomyces cerevisiae
Genetics
186
561-571
2010
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691507
Kemmer
Combining chemical genomics sc ...
Saccharomyces cerevisiae
BMC Microbiol.
9
9-9
2009
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691983
Drenckhahn
Compensatory growth of healthy ...
Mus musculus
Dev. Cell
15
521-533
2008
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682001
Hartshorne
A dedicated haem lyase is requ ...
Wolinella succinogenes
Mol. Microbiol.
64
1049-1060
2007
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677440
Wimplinger
Mutations of the mitochondrial ...
Homo sapiens
Am. J. Hum. Genet.
79
878-889
2006
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679489
Kiryu-Seo
Unique anti-apoptotic activity ...
Mus musculus
EMBO J.
25
3411-3421
2006
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3
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1
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665678
Bernard
Cyc2p, a membrane-bound flavop ...
Saccharomyces cerevisiae
J. Biol. Chem.
280
39852-39859
2005
1
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3
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652487
Bernard
Overlapping specificities of t ...
Homo sapiens, Mus musculus, Saccharomyces cerevisiae
J. Biol. Chem.
278
49732-49742
2003
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3
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6
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6
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3
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6
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652352
Ren
A bacterial cytochrome c heme ...
Escherichia coli
J. Biol. Chem.
277
7657-7663
2002
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1
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651895
Tong
Cytochrome c heme lyase activi ...
Saccharomyces cerevisiae
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