BRENDA - Enzyme Database
show all sequences of 4.4.1.17

Substrate recognition of holocytochrome c synthase: N-terminal region and CXXCH motif of mitochondrial cytochrome c

Zhang, Y.; Stevens, J.M.; Ferguson, S.J.; FEBS Lett. 588, 3367-3374 (2014)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
coexpression of the wild-type enzyme with periplasmic targeting sequence and addition of the Pseudomonas aeruginosa cytochrome c551/552 pre-sequence, and mutant enzymes with Equus caballus heart cytochrome c or Strep-tagged Saccharomyces cerevisiaecytochrome c in Escherichia coli strain BL21(DE3)
Saccharomyces cerevisiae
Engineering
Protein Variants
Commentary
Organism
C15A
site-directed mutagenesis, cytoplasm-targeted mutant, the mutant is matured and undergoes heme attachment like the wild-type enzyme
Saccharomyces cerevisiae
C18A
site-directed mutagenesis, cytoplasm-targeted mutant, the mutant does not undergo heme attachment
Saccharomyces cerevisiae
F15A
site-directed mutagenesis, periplasm-targeted mutant, the mutant variant does not produce holocytochrome c
Saccharomyces cerevisiae
F15E
site-directed mutagenesis, periplasm-targeted mutant, the mutant variant does not produce holocytochrome c
Saccharomyces cerevisiae
F15I
site-directed mutagenesis, periplasm-targeted mutant, the mutant shows reduced holocytochrome c production compared to the wild-type enzyme
Saccharomyces cerevisiae
F15W
site-directed mutagenesis, periplasm-targeted mutant, the mutant shows reduced holocytochrome c production compared to the wild-type enzyme
Saccharomyces cerevisiae
F15Y
site-directed mutagenesis, periplasm-targeted mutant, the mutant shows unaltered holocytochrome c production like the wild-type enzyme
Saccharomyces cerevisiae
H19K
site-directed mutagenesis, cytoplasm-targeted mutant, the mutant does not undergo heme attachment
Saccharomyces cerevisiae
H19M
site-directed mutagenesis, cytoplasm-targeted mutant, the mutant does not undergo heme attachment
Saccharomyces cerevisiae
H19R
site-directed mutagenesis, cytoplasm-targeted mutant, the mutant does not undergo heme attachment
Saccharomyces cerevisiae
additional information
construction of T17, K16, G29, H45, and K60 deletion mutants and of a mutant with periplasmic targeting sequence
Saccharomyces cerevisiae
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
-
Saccharomyces cerevisiae
5739
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
Apocytochrome c + heme
Saccharomyces cerevisiae
-
Holocytochrome c
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Saccharomyces cerevisiae
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
Apocytochrome c + heme
-
729740
Saccharomyces cerevisiae
Holocytochrome c
-
-
-
?
Apocytochrome c + heme
Equus caballus heart cytochrome c or Strep-tagged Saccharomyces cerevisiae cytochrome c are coexpressed in Escherichia coli strain BL21(DE3) with Saccharomyces cerevisiae holocytochrome c synthase. No product synthesis from truncated cytochrome c1 mutants G29X, H45X and K60X, with the X indicating the position of the inserted stop codon
729740
Saccharomyces cerevisiae
Holocytochrome c
-
-
-
?
additional information
the CXXCH motif and the N-terminus of the apocytochrome polypeptide are important protein-protein recognition motifs in enzyme-substrate interaction, the N-terminal region of the mitochondrial cytochrome c sequence is critical for attachment of heme to apocytochrome c
729740
Saccharomyces cerevisiae
?
-
-
-
-
Synonyms
Synonyms
Commentary
Organism
HCC synthase
-
Saccharomyces cerevisiae
HCCS
-
Saccharomyces cerevisiae
holocytochrome c synthase
-
Saccharomyces cerevisiae
Cloned(Commentary) (protein specific)
Commentary
Organism
coexpression of the wild-type enzyme with periplasmic targeting sequence and addition of the Pseudomonas aeruginosa cytochrome c551/552 pre-sequence, and mutant enzymes with Equus caballus heart cytochrome c or Strep-tagged Saccharomyces cerevisiaecytochrome c in Escherichia coli strain BL21(DE3)
Saccharomyces cerevisiae
Engineering (protein specific)
Protein Variants
Commentary
Organism
C15A
site-directed mutagenesis, cytoplasm-targeted mutant, the mutant is matured and undergoes heme attachment like the wild-type enzyme
Saccharomyces cerevisiae
C18A
site-directed mutagenesis, cytoplasm-targeted mutant, the mutant does not undergo heme attachment
Saccharomyces cerevisiae
F15A
site-directed mutagenesis, periplasm-targeted mutant, the mutant variant does not produce holocytochrome c
Saccharomyces cerevisiae
F15E
site-directed mutagenesis, periplasm-targeted mutant, the mutant variant does not produce holocytochrome c
Saccharomyces cerevisiae
F15I
site-directed mutagenesis, periplasm-targeted mutant, the mutant shows reduced holocytochrome c production compared to the wild-type enzyme
Saccharomyces cerevisiae
F15W
site-directed mutagenesis, periplasm-targeted mutant, the mutant shows reduced holocytochrome c production compared to the wild-type enzyme
Saccharomyces cerevisiae
F15Y
site-directed mutagenesis, periplasm-targeted mutant, the mutant shows unaltered holocytochrome c production like the wild-type enzyme
Saccharomyces cerevisiae
H19K
site-directed mutagenesis, cytoplasm-targeted mutant, the mutant does not undergo heme attachment
Saccharomyces cerevisiae
H19M
site-directed mutagenesis, cytoplasm-targeted mutant, the mutant does not undergo heme attachment
Saccharomyces cerevisiae
H19R
site-directed mutagenesis, cytoplasm-targeted mutant, the mutant does not undergo heme attachment
Saccharomyces cerevisiae
additional information
construction of T17, K16, G29, H45, and K60 deletion mutants and of a mutant with periplasmic targeting sequence
Saccharomyces cerevisiae
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
-
Saccharomyces cerevisiae
5739
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
Apocytochrome c + heme
Saccharomyces cerevisiae
-
Holocytochrome c
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
Apocytochrome c + heme
-
729740
Saccharomyces cerevisiae
Holocytochrome c
-
-
-
?
Apocytochrome c + heme
Equus caballus heart cytochrome c or Strep-tagged Saccharomyces cerevisiae cytochrome c are coexpressed in Escherichia coli strain BL21(DE3) with Saccharomyces cerevisiae holocytochrome c synthase. No product synthesis from truncated cytochrome c1 mutants G29X, H45X and K60X, with the X indicating the position of the inserted stop codon
729740
Saccharomyces cerevisiae
Holocytochrome c
-
-
-
?
additional information
the CXXCH motif and the N-terminus of the apocytochrome polypeptide are important protein-protein recognition motifs in enzyme-substrate interaction, the N-terminal region of the mitochondrial cytochrome c sequence is critical for attachment of heme to apocytochrome c
729740
Saccharomyces cerevisiae
?
-
-
-
-
Other publictions for EC 4.4.1.17
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
747125
Babbitt
Biosynthesis of single thioet ...
Homo sapiens
Biochemistry
56
3337-3346
2017
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749183
Mendez
Engineered holocytochrome c s ...
Homo sapiens
Proc. Natl. Acad. Sci. USA
114
2235-2240
2017
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1
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748211
Babbitt
Molecular basis behind inabil ...
Homo sapiens
J. Biol. Chem.
291
17523-17534
2016
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1
1
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729264
Babbitt
Conserved residues of the huma ...
Homo sapiens
Biochemistry
53
5261-5271
2014
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1
-
12
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2
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1
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12
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2
2
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729740
Zhang
Substrate recognition of holoc ...
Saccharomyces cerevisiae
FEBS Lett.
588
3367-3374
2014
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1
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11
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3
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11
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730058
Babbitt
Mechanisms of mitochondrial ho ...
Homo sapiens
J. Biol. Chem.
289
28795-18807
2014
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1
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730827
San Francisco
Human mitochondrial holocytoch ...
Homo sapiens
Proc. Natl. Acad. Sci. USA
110
E788-E797
2013
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715034
Stevens
The mitochondrial cytochrome c ...
Saccharomyces cerevisiae
FEBS Lett.
585
1891-1896
2011
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729727
Moore
Mitochondrial cytochrome c syn ...
Saccharomyces cerevisiae
FEBS Lett.
585
3415-3419
2011
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9
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3
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7
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9
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1
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730322
Kleingardner
Comparing substrate specificit ...
Saccharomyces cerevisiae
Metallomics
3
396-403
2011
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1
1
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6
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2
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713714
Qidwai
Deletions of Xp provide eviden ...
Homo sapiens
Am. J. Med. Genet. A
152
1588-1590
2010
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1
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715120
Corvest
c-type cytochrome assembly in ...
Saccharomyces cerevisiae
Genetics
186
561-571
2010
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691507
Kemmer
Combining chemical genomics sc ...
Saccharomyces cerevisiae
BMC Microbiol.
9
9-9
2009
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691983
Drenckhahn
Compensatory growth of healthy ...
Mus musculus
Dev. Cell
15
521-533
2008
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682001
Hartshorne
A dedicated haem lyase is requ ...
Wolinella succinogenes
Mol. Microbiol.
64
1049-1060
2007
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677440
Wimplinger
Mutations of the mitochondrial ...
Homo sapiens
Am. J. Hum. Genet.
79
878-889
2006
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679489
Kiryu-Seo
Unique anti-apoptotic activity ...
Mus musculus
EMBO J.
25
3411-3421
2006
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665678
Bernard
Cyc2p, a membrane-bound flavop ...
Saccharomyces cerevisiae
J. Biol. Chem.
280
39852-39859
2005
1
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652487
Bernard
Overlapping specificities of t ...
Homo sapiens, Mus musculus, Saccharomyces cerevisiae
J. Biol. Chem.
278
49732-49742
2003
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3
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6
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652352
Ren
A bacterial cytochrome c heme ...
Escherichia coli
J. Biol. Chem.
277
7657-7663
2002
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Localization of enzyme for hem ...
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