BRENDA - Enzyme Database
show all sequences of 4.4.1.17

Mitochondrial cytochrome c synthase: CP motifs are not necessary for heme attachment to apocytochrome c

Moore, R.L.; Stevens, J.M.; Ferguson, S.J.; FEBS Lett. 585, 3415-3419 (2011)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
recombinant expression of His-tagged wild-type enzyme and mutants in Escherichia coli strain BL21(DE3) cytoplasm, coexpression with Equus caballus cytochrome c
Saccharomyces cerevisiae
Engineering
Protein Variants
Commentary
Organism
C26A
site-directed mutagenesis of CP-motif 1 of the enzyme, the mutant shows activity similar to the wild-type enzyme
Saccharomyces cerevisiae
C26A/C42A
site-directed mutagenesis of both CP-motifs of the enzyme, the mutant shows activity similar to the wild-type enzyme
Saccharomyces cerevisiae
C26A/P27A
site-directed mutagenesis of CP-motif 1 of the enzyme, the mutant shows activity similar to the wild-type enzyme
Saccharomyces cerevisiae
C42A
site-directed mutagenesis of CP-motif 2 of the enzyme, the mutant shows activity similar to the wild-type enzyme
Saccharomyces cerevisiae
C42A/P43A
site-directed mutagenesis of CP-motif 2 of the enzyme, the mutant shows activity similar to the wild-type enzyme
Saccharomyces cerevisiae
E133A
site-directed mutagenesis, the mutant shows highly reduced activity compared with the wild-type enzyme
Saccharomyces cerevisiae
E133K
site-directed mutagenesis, the mutant shows highly reduced activity compared with the wild-type enzyme
Saccharomyces cerevisiae
R199A
site-directed mutagenesis, almost inactive mutant
Saccharomyces cerevisiae
R199C
site-directed mutagenesis, almost inactive mutant
Saccharomyces cerevisiae
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
Apocytochrome c + heme
Saccharomyces cerevisiae
-
Holocytochrome c
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Saccharomyces cerevisiae
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
Apocytochrome c + heme
-
729727
Saccharomyces cerevisiae
Holocytochrome c
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
HCCS
-
Saccharomyces cerevisiae
heme lyase
-
Saccharomyces cerevisiae
holocytochrome c synthase
-
Saccharomyces cerevisiae
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Saccharomyces cerevisiae
Cloned(Commentary) (protein specific)
Commentary
Organism
recombinant expression of His-tagged wild-type enzyme and mutants in Escherichia coli strain BL21(DE3) cytoplasm, coexpression with Equus caballus cytochrome c
Saccharomyces cerevisiae
Engineering (protein specific)
Protein Variants
Commentary
Organism
C26A
site-directed mutagenesis of CP-motif 1 of the enzyme, the mutant shows activity similar to the wild-type enzyme
Saccharomyces cerevisiae
C26A/C42A
site-directed mutagenesis of both CP-motifs of the enzyme, the mutant shows activity similar to the wild-type enzyme
Saccharomyces cerevisiae
C26A/P27A
site-directed mutagenesis of CP-motif 1 of the enzyme, the mutant shows activity similar to the wild-type enzyme
Saccharomyces cerevisiae
C42A
site-directed mutagenesis of CP-motif 2 of the enzyme, the mutant shows activity similar to the wild-type enzyme
Saccharomyces cerevisiae
C42A/P43A
site-directed mutagenesis of CP-motif 2 of the enzyme, the mutant shows activity similar to the wild-type enzyme
Saccharomyces cerevisiae
E133A
site-directed mutagenesis, the mutant shows highly reduced activity compared with the wild-type enzyme
Saccharomyces cerevisiae
E133K
site-directed mutagenesis, the mutant shows highly reduced activity compared with the wild-type enzyme
Saccharomyces cerevisiae
R199A
site-directed mutagenesis, almost inactive mutant
Saccharomyces cerevisiae
R199C
site-directed mutagenesis, almost inactive mutant
Saccharomyces cerevisiae
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
Apocytochrome c + heme
Saccharomyces cerevisiae
-
Holocytochrome c
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
Apocytochrome c + heme
-
729727
Saccharomyces cerevisiae
Holocytochrome c
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Saccharomyces cerevisiae
General Information
General Information
Commentary
Organism
additional information
the CP motifs, commonly found in heme-binding proteins, are not required for enzyme activity of te Saccharomyyces cerevisiae enzyme, while mutations in the human enzyme on the C-terminal side of the CP motifs cause disease states of microphthalmia with linear skin defects due to abolished or drastically attenuated holocytochrome c production
Saccharomyces cerevisiae
General Information (protein specific)
General Information
Commentary
Organism
additional information
the CP motifs, commonly found in heme-binding proteins, are not required for enzyme activity of te Saccharomyyces cerevisiae enzyme, while mutations in the human enzyme on the C-terminal side of the CP motifs cause disease states of microphthalmia with linear skin defects due to abolished or drastically attenuated holocytochrome c production
Saccharomyces cerevisiae
Other publictions for EC 4.4.1.17
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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Engineered holocytochrome c s ...
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114
2235-2240
2017
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1
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748211
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J. Biol. Chem.
291
17523-17534
2016
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1
1
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Homo sapiens
Biochemistry
53
5261-5271
2014
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1
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12
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2
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1
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2
2
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729740
Zhang
Substrate recognition of holoc ...
Saccharomyces cerevisiae
FEBS Lett.
588
3367-3374
2014
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1
-
11
-
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1
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1
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730058
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Homo sapiens
J. Biol. Chem.
289
28795-18807
2014
-
-
1
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2
-
-
-
1
-
-
1
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3
-
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730827
San Francisco
Human mitochondrial holocytoch ...
Homo sapiens
Proc. Natl. Acad. Sci. USA
110
E788-E797
2013
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715034
Stevens
The mitochondrial cytochrome c ...
Saccharomyces cerevisiae
FEBS Lett.
585
1891-1896
2011
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729727
Moore
Mitochondrial cytochrome c syn ...
Saccharomyces cerevisiae
FEBS Lett.
585
3415-3419
2011
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9
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730322
Kleingardner
Comparing substrate specificit ...
Saccharomyces cerevisiae
Metallomics
3
396-403
2011
-
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1
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6
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-
2
2
-
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713714
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Deletions of Xp provide eviden ...
Homo sapiens
Am. J. Med. Genet. A
152
1588-1590
2010
-
1
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-
-
-
-
-
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1
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715120
Corvest
c-type cytochrome assembly in ...
Saccharomyces cerevisiae
Genetics
186
561-571
2010
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691507
Kemmer
Combining chemical genomics sc ...
Saccharomyces cerevisiae
BMC Microbiol.
9
9-9
2009
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691983
Drenckhahn
Compensatory growth of healthy ...
Mus musculus
Dev. Cell
15
521-533
2008
-
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-
-
-
-
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-
-
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-
-
2
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1
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2
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682001
Hartshorne
A dedicated haem lyase is requ ...
Wolinella succinogenes
Mol. Microbiol.
64
1049-1060
2007
-
-
-
-
1
-
-
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-
-
-
-
-
1
-
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677440
Wimplinger
Mutations of the mitochondrial ...
Homo sapiens
Am. J. Hum. Genet.
79
878-889
2006
-
1
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2
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1
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3
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679489
Kiryu-Seo
Unique anti-apoptotic activity ...
Mus musculus
EMBO J.
25
3411-3421
2006
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1
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1
-
3
-
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665678
Bernard
Cyc2p, a membrane-bound flavop ...
Saccharomyces cerevisiae
J. Biol. Chem.
280
39852-39859
2005
1
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1
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-
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652487
Bernard
Overlapping specificities of t ...
Homo sapiens, Mus musculus, Saccharomyces cerevisiae
J. Biol. Chem.
278
49732-49742
2003
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3
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6
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652352
Ren
A bacterial cytochrome c heme ...
Escherichia coli
J. Biol. Chem.
277
7657-7663
2002
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651895
Tong
Cytochrome c heme lyase activi ...
Saccharomyces cerevisiae
J. Biol. Chem.
273
25695-25702
1998
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1
2
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653258
Cervera
Molecular cloning and characte ...
Candida albicans
Mol. Microbiol.
30
67-81
1998
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1
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1
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-
-
-
-
-
-
-
-
-
-
-
651429
Schaefer
Cloning and characterization o ...
Homo sapiens, Mus musculus
Genomics
34
166-172
1996
-
-
2
-
-
-
-
-
-
-
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-
2
-
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
651850
Steiner
Biogenesis of mitochondrial he ...
Saccharomyces cerevisiae
J. Biol. Chem.
270
22842-22849
1995
-
-
1
-
-
-
-
-
1
-
-
-
-
2
-
-
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-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
94058
Lill
Import of cytochrome c heme ly ...
Neurospora crassa
EMBO J.
11
449-456
1992
-
-
-
-
-
-
-
-
4
-
-
1
-
2
-
-
1
-
-
1
-
-
2
-
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-
-
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4
-
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1
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1
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
94059
Zollner
Molecular cloning and characte ...
Neurospora crassa, Saccharomyces cerevisiae
Eur. J. Biochem.
207
1093-1100
1992
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
4
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1
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-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
94060
Drygas
Cloning and analysis of the Ne ...
Neurospora crassa
J. Biol. Chem.
264
17897-17906
1989
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
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1
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-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
94056
Dumont
Identification and sequence of ...
Saccharomyces cerevisiae
EMBO J.
6
235-241
1987
-
-
1
-
-
-
-
-
2
-
1
-
-
2
-
-
-
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3
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1
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-
-
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
94057
Nicholson
Import of cytochrome c into mi ...
Neurospora crassa
Eur. J. Biochem.
164
147-157
1987
5
-
-
-
-
-
3
-
3
-
-
-
-
2
-
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1
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1
1
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3
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5
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1
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3
-
1
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1
1
-
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-
-
-
-
-
-
94055
Enosawa
Localization of enzyme for hem ...
Saccharomyces cerevisiae
Biochem. Biophys. Res. Commun.
141
1145-1150
1986
-
-
-
-
-
-
-
-
3
-
-
1
-
1
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1
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