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Literature summary for 4.4.1.16 extracted from
- Selenocysteine beta-lyase (porcine) (1987), Methods Enzymol., 143, 415-418.
General Stability
| General Stability | Organism |
|---|---|
| 1% bovine serum albumin, 20% sucrose or 20% glycerol protects against inactivation, highly diluted samples sensitive to freezing | Sus scrofa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| L-cysteine | - |
Sus scrofa |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.83 | - |
L-selenocysteine | pH 8.5, 37°C | Sus scrofa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
SDS-PAGE | Sus scrofa |
| 85000 | - |
sedimentation equilibrium centrifugation | Sus scrofa |
| 93000 | - |
gel filtration | Sus scrofa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-selenocysteine + reduced acceptor | Sus scrofa | - |
selenide + L-alanine + acceptor | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sus scrofa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sus scrofa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Sus scrofa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 37 | - |
purified enzyme, pH 8.5, 37°C | Sus scrofa |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 20% sucrose, stable | Sus scrofa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-selenocysteine + reduced acceptor | - |
Sus scrofa | selenide + L-alanine + acceptor | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 48000, SDS-PAGE | Sus scrofa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | required | Sus scrofa | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1 | - |
L-cysteine | pH 8.5, 37°C | Sus scrofa | |
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