BRENDA - Enzyme Database show
show all sequences of 4.4.1.15

Isolation and characterization of a D-cysteine desulfhydrase protein from Arabidopsis thaliana

Riemenschneider, A.; Wegele, R.; Schmidt, A.; Papenbrock, J.; FEBS J. 272, 1291-1304 (2005)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Arabidopsis thaliana
General Stability
General Stability
Organism
glycerol, pyridoxal-5'-phosphate, dithiothreitol and EDTA do not increase the stability of the protein after freezing
Arabidopsis thaliana
Inhibitors
Inhibitors
Commentary
Organism
Structure
aminooxy acetic acid
at 10 microM, IC50 value is 0.0305 mM
Arabidopsis thaliana
hydroxylamine
at 10 microM, IC50 value is 0.0159 mM
Arabidopsis thaliana
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.25
-
D-cysteine
purified recombinant mature protein
Arabidopsis thaliana
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
-
Arabidopsis thaliana
5739
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
41700
-
monomer, mature protein without N-terminal extension, calculated from amino acid sequence
Arabidopsis thaliana
42000
-
x * 42000, SDS-PAGE, unprocessed protein
Arabidopsis thaliana
43000
-
x * 43000, SDS-PAGE, mature protein
Arabidopsis thaliana
43900
-
monomer, calculated from amino acid sequence
Arabidopsis thaliana
44000
-
x * 44000, SDS-PAGE, post-translationally modified protein
Arabidopsis thaliana
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-cysteine + H2O
Arabidopsis thaliana
-
sulfide + NH3 + pyruvate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
-
ecotype C24
-
Purification (Commentary)
Commentary
Organism
recombinant protein
Arabidopsis thaliana
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-cysteine + H2O
-
664881
Arabidopsis thaliana
sulfide + NH3 + pyruvate
-
-
-
?
D-cysteine + H2O
37°C, pH 8.0
664881
Arabidopsis thaliana
sulfide + NH3 + pyruvate
-
-
-
?
L-cysteine + H2O
5% activity compared to D-cysteine
664881
Arabidopsis thaliana
sulfide + NH3 + pyruvate
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 42000, SDS-PAGE, unprocessed protein; x * 43000, SDS-PAGE, mature protein; x * 44000, SDS-PAGE, post-translationally modified protein
Arabidopsis thaliana
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
additional information
-
loss of activity at 50°C, no activity at 60°C. The enzyme is also very sensitive to freezing. One freeze-thaw cycle leads to a loss of activity of 75%.
Arabidopsis thaliana
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
6
-
D-cysteine
-
Arabidopsis thaliana
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Arabidopsis thaliana
Cofactor
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
-
Arabidopsis thaliana
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Arabidopsis thaliana
mature protein without N-terminal extension, calculated from amino acid sequence
-
4.34
Arabidopsis thaliana
calculated from amino acid sequence
-
7.2
IC50 Value
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.0159
-
at 10 microM, IC50 value is 0.0159 mM
Arabidopsis thaliana
hydroxylamine
0.0305
-
at 10 microM, IC50 value is 0.0305 mM
Arabidopsis thaliana
aminooxy acetic acid
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Arabidopsis thaliana
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
-
Arabidopsis thaliana
General Stability (protein specific)
General Stability
Organism
glycerol, pyridoxal-5'-phosphate, dithiothreitol and EDTA do not increase the stability of the protein after freezing
Arabidopsis thaliana
IC50 Value (protein specific)
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.0159
-
at 10 microM, IC50 value is 0.0159 mM
Arabidopsis thaliana
hydroxylamine
0.0305
-
at 10 microM, IC50 value is 0.0305 mM
Arabidopsis thaliana
aminooxy acetic acid
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
aminooxy acetic acid
at 10 microM, IC50 value is 0.0305 mM
Arabidopsis thaliana
hydroxylamine
at 10 microM, IC50 value is 0.0159 mM
Arabidopsis thaliana
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.25
-
D-cysteine
purified recombinant mature protein
Arabidopsis thaliana
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
-
Arabidopsis thaliana
5739
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
41700
-
monomer, mature protein without N-terminal extension, calculated from amino acid sequence
Arabidopsis thaliana
42000
-
x * 42000, SDS-PAGE, unprocessed protein
Arabidopsis thaliana
43000
-
x * 43000, SDS-PAGE, mature protein
Arabidopsis thaliana
43900
-
monomer, calculated from amino acid sequence
Arabidopsis thaliana
44000
-
x * 44000, SDS-PAGE, post-translationally modified protein
Arabidopsis thaliana
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-cysteine + H2O
Arabidopsis thaliana
-
sulfide + NH3 + pyruvate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant protein
Arabidopsis thaliana
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-cysteine + H2O
-
664881
Arabidopsis thaliana
sulfide + NH3 + pyruvate
-
-
-
?
D-cysteine + H2O
37°C, pH 8.0
664881
Arabidopsis thaliana
sulfide + NH3 + pyruvate
-
-
-
?
L-cysteine + H2O
5% activity compared to D-cysteine
664881
Arabidopsis thaliana
sulfide + NH3 + pyruvate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 42000, SDS-PAGE, unprocessed protein; x * 43000, SDS-PAGE, mature protein; x * 44000, SDS-PAGE, post-translationally modified protein
Arabidopsis thaliana
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
additional information
-
loss of activity at 50°C, no activity at 60°C. The enzyme is also very sensitive to freezing. One freeze-thaw cycle leads to a loss of activity of 75%.
Arabidopsis thaliana
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
6
-
D-cysteine
-
Arabidopsis thaliana
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Arabidopsis thaliana
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Arabidopsis thaliana
mature protein without N-terminal extension, calculated from amino acid sequence
-
4.34
Arabidopsis thaliana
calculated from amino acid sequence
-
7.2
Other publictions for EC 4.4.1.15
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746770
Ekimova
Distribution of 1-aminocyclop ...
Methylorubrum extorquens, Methylorubrum extorquens DSM 1338
Antonie van Leeuwenhoek
111
1723-1734
2018
-
-
-
-
-
-
-
1
-
-
1
-
-
4
-
-
-
-
-
-
-
-
4
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
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-
-
-
-
-
-
-
4
-
1
-
-
1
1
-
-
-
-
-
-
-
1
1
748473
Li
-
Identification of wheat D-cys ...
Triticum aestivum
J. Plant Growth Regul.
37
1175-1184
2018
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
730698
Bharath
Structural and mutational stud ...
Salmonella enterica subsp. enterica serovar Typhimurium
PLoS ONE
7
e36267
2012
-
-
1
1
7
-
-
1
-
-
-
1
-
3
-
-
1
-
-
-
-
-
3
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
7
-
-
-
-
1
-
-
-
1
-
-
-
1
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
2
2
-
-
-
729125
Jin
Hydrogen sulfide improves drou ...
Arabidopsis thaliana, Arabidopsis thaliana Col-0
Biochem. Biophys. Res. Commun.
414
481-486
2011
-
-
1
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
2
2
2
2
-
-
694780
Todorovic
The interconversion of ACC dea ...
Pseudomonas putida, Pseudomonas putida UW4, Solanum lycopersicum
Planta
229
193-205
2008
-
-
2
-
4
-
2
2
-
-
1
6
-
7
-
-
2
-
-
2
6
-
6
-
2
-
1
2
2
-
-
-
1
-
-
-
-
2
-
-
4
-
-
2
1
2
-
-
1
6
-
-
-
2
-
2
6
-
6
-
2
-
1
2
2
-
-
-
-
-
-
-
-
-
680893
Tarze
Extracellular production of hy ...
Escherichia coli
J. Biol. Chem.
282
8759-8767
2007
1
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
1
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
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-
1
-
-
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
680123
Jain
Catalytic and regulatory prope ...
Synechococcus elongatus
Indian J. Exp. Biol.
44
767-772
2006
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
8
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
8
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
664881
Riemenschneider
Isolation and characterization ...
Arabidopsis thaliana
FEBS J.
272
1291-1304
2005
-
-
1
-
-
1
2
1
1
-
5
1
-
4
-
-
1
-
-
-
-
-
3
1
-
1
-
1
1
-
-
1
-
2
2
-
-
1
1
-
-
1
2
2
-
1
1
-
5
1
-
-
-
1
-
-
-
-
3
1
-
1
-
1
1
-
-
2
-
-
-
-
-
-
648739
Soutourina
Role of D-cysteine desulfhydra ...
Escherichia coli
J. Biol. Chem.
276
40864-40872
2001
-
-
1
-
-
-
-
2
-
-
2
1
-
3
-
-
1
-
-
-
-
-
4
-
-
-
-
2
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
2
-
-
2
1
-
-
-
1
-
-
-
-
4
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
645577
Bagchi
-
Partial purification and regul ...
Phormidium uncinatum, Phormidium uncinatum CU 1462/7
Indian J. Exp. Biol.
35
876-880
1997
-
-
-
-
-
-
-
-
-
-
-
2
-
3
-
-
1
-
-
-
-
-
2
-
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2
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1
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-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648737
Schuetz
-
Thiol accumulation and cystein ...
Cucurbita pepo
Plant Cell Physiol.
32
733-736
1991
-
-
-
-
-
-
3
-
-
-
-
1
-
1
-
-
-
-
-
2
-
-
1
-
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3
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1
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2
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1
-
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-
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-
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-
-
34752
Nagasawa
Physiological comparison of D- ...
Escherichia coli, Escherichia coli W3110 / ATCC 27325, Pseudomonas putida, Pseudomonas putida CR 1-1
Arch. Microbiol.
149
413-416
1988
-
-
-
-
-
-
-
-
2
-
-
4
-
32
-
-
-
-
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-
-
-
12
-
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2
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4
-
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12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648734
Schmidt
-
D-cysteine desulfhydrase from ...
Spinacia oleracea
Methods Enzymol.
143
449-453
1987
1
-
-
-
-
-
4
-
-
-
-
2
-
1
-
-
1
-
-
1
1
-
5
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
1
-
-
-
-
4
-
-
-
-
-
2
-
-
-
1
-
1
1
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648735
Rennenberg
-
Cysteine desulphydrase activit ...
Cucurbita pepo, Nicotiana tabacum
Phytochemistry
26
1583-1589
1987
-
-
-
-
-
-
4
-
2
-
-
2
-
2
-
-
-
-
-
2
-
-
4
-
-
-
-
-
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-
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4
-
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2
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-
2
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-
-
2
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648731
Nagasawa
D-Cysteine desulfhydrase of Es ...
Citrobacter freundii, Enterobacter cloacae, Escherichia coli, Escherichia coli W3110 / ATCC 27325, Klebsiella pneumoniae
Eur. J. Biochem.
153
541-551
1985
-
-
-
-
-
-
10
13
-
-
4
5
-
33
-
-
1
-
-
-
2
1
29
1
1
-
4
-
2
1
-
1
1
-
-
-
-
-
1
-
-
-
-
10
1
13
-
-
4
5
-
-
-
1
-
-
2
1
29
1
1
-
4
-
2
1
-
-
-
-
-
-
-
-
648733
Schmidt
-
A cysteine desulfhydrase speci ...
Chlorella fusca
Z. Naturforsch. C
38 c
428-435
1983
-
-
-
-
-
-
3
1
-
-
-
2
-
1
-
-
1
-
-
-
1
-
5
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
1
-
-
-
2
-
-
-
1
-
-
1
-
5
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
648732
Schmidt
A cysteine desulfhydrase from ...
Spinacia oleracea
Z. Pflanzenphysiol.
107
295-300
1982
-
-
-
-
-
-
3
1
-
-
1
1
-
1
-
-
1
-
-
1
-
1
2
-
-
-
-
-
1
1
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-
-
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-
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-
-
-
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-
-
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3
-
1
-
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1
1
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-
1
-
1
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1
2
-
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-
-
-
1
1
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-