BRENDA - Enzyme Database show
show all sequences of 4.4.1.15

D-cysteine desulfhydrase from spinach

Schmidt, A.; Methods Enzymol. 143, 449-453 (1987)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
dithiothreitol
probably by preventing autooxidation of substrate
Spinacia oleracea
Inhibitors
Inhibitors
Commentary
Organism
Structure
aminooxyacetic acid
-
Spinacia oleracea
Co2+
-
Spinacia oleracea
hydroxylamine
-
Spinacia oleracea
Ni2+
-
Spinacia oleracea
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-cysteine + H2O
Spinacia oleracea
plant sulfur metabolism
sulfide + NH3 + pyruvate
additional product suggested
Spinacia oleracea
?
D-cysteine + H2O
Spinacia oleracea
plant sulfur metabolism
sulfide + NH3 + pyruvate
more sulfide than pyruvate and ammonium formed
Spinacia oleracea
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Spinacia oleracea
-
-
-
Purification (Commentary)
Commentary
Organism
-
Spinacia oleracea
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Spinacia oleracea
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
0.01
-
purified enzyme, 37░C, pH 9.0
Spinacia oleracea
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-cysteine + H2O
very specific, no activity with L-cysteine, mercaptoacetic acid, mercaptoethanol, D-cystine, L-cystine, cysteamine, L-cysteine methylester and dithioerythritol
648734
Spinacia oleracea
sulfide + NH3 + pyruvate
additional product suggested
648734
Spinacia oleracea
?
D-cysteine + H2O
very specific, no activity with L-cysteine, mercaptoacetic acid, mercaptoethanol, D-cystine, L-cystine, cysteamine, L-cysteine methylester and dithioerythritol
648734
Spinacia oleracea
sulfide + NH3 + pyruvate
more sulfide than pyruvate and ammonium formed
648734
Spinacia oleracea
?
D-cysteine + H2O
plant sulfur metabolism
648734
Spinacia oleracea
sulfide + NH3 + pyruvate
additional product suggested
648734
Spinacia oleracea
?
D-cysteine + H2O
plant sulfur metabolism
648734
Spinacia oleracea
sulfide + NH3 + pyruvate
more sulfide than pyruvate and ammonium formed
648734
Spinacia oleracea
?
additional information
L-cysteine is not utilized
648734
Spinacia oleracea
?
-
-
-
-
Cofactor
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
-
Spinacia oleracea
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
dithiothreitol
probably by preventing autooxidation of substrate
Spinacia oleracea
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
-
Spinacia oleracea
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
aminooxyacetic acid
-
Spinacia oleracea
Co2+
-
Spinacia oleracea
hydroxylamine
-
Spinacia oleracea
Ni2+
-
Spinacia oleracea
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-cysteine + H2O
Spinacia oleracea
plant sulfur metabolism
sulfide + NH3 + pyruvate
additional product suggested
Spinacia oleracea
?
D-cysteine + H2O
Spinacia oleracea
plant sulfur metabolism
sulfide + NH3 + pyruvate
more sulfide than pyruvate and ammonium formed
Spinacia oleracea
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Spinacia oleracea
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Spinacia oleracea
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
0.01
-
purified enzyme, 37░C, pH 9.0
Spinacia oleracea
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-cysteine + H2O
very specific, no activity with L-cysteine, mercaptoacetic acid, mercaptoethanol, D-cystine, L-cystine, cysteamine, L-cysteine methylester and dithioerythritol
648734
Spinacia oleracea
sulfide + NH3 + pyruvate
additional product suggested
648734
Spinacia oleracea
?
D-cysteine + H2O
very specific, no activity with L-cysteine, mercaptoacetic acid, mercaptoethanol, D-cystine, L-cystine, cysteamine, L-cysteine methylester and dithioerythritol
648734
Spinacia oleracea
sulfide + NH3 + pyruvate
more sulfide than pyruvate and ammonium formed
648734
Spinacia oleracea
?
D-cysteine + H2O
plant sulfur metabolism
648734
Spinacia oleracea
sulfide + NH3 + pyruvate
additional product suggested
648734
Spinacia oleracea
?
D-cysteine + H2O
plant sulfur metabolism
648734
Spinacia oleracea
sulfide + NH3 + pyruvate
more sulfide than pyruvate and ammonium formed
648734
Spinacia oleracea
?
additional information
L-cysteine is not utilized
648734
Spinacia oleracea
?
-
-
-
-
Other publictions for EC 4.4.1.15
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746770
Ekimova
Distribution of 1-aminocyclop ...
Methylorubrum extorquens, Methylorubrum extorquens DSM 1338
Antonie van Leeuwenhoek
111
1723-1734
2018
-
-
-
-
-
-
-
1
-
-
1
-
-
4
-
-
-
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4
-
1
-
-
1
1
-
-
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-
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-
-
1
-
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1
-
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-
-
-
-
-
-
-
4
-
1
-
-
1
1
-
-
-
-
-
-
-
1
1
748473
Li
-
Identification of wheat D-cys ...
Triticum aestivum
J. Plant Growth Regul.
37
1175-1184
2018
-
-
-
-
-
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-
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1
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-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
730698
Bharath
Structural and mutational stud ...
Salmonella enterica subsp. enterica serovar Typhimurium
PLoS ONE
7
e36267
2012
-
-
1
1
7
-
-
1
-
-
-
1
-
3
-
-
1
-
-
-
-
-
3
1
-
-
-
-
-
-
-
1
-
-
-
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-
1
1
1
7
-
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1
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-
1
-
-
-
1
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
2
2
-
-
-
729125
Jin
Hydrogen sulfide improves drou ...
Arabidopsis thaliana, Arabidopsis thaliana Col-0
Biochem. Biophys. Res. Commun.
414
481-486
2011
-
-
1
-
-
-
-
-
-
-
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-
7
-
-
-
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-
7
-
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-
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-
1
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-
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-
1
1
-
-
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-
-
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-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
2
2
2
2
-
-
694780
Todorovic
The interconversion of ACC dea ...
Pseudomonas putida, Pseudomonas putida UW4, Solanum lycopersicum
Planta
229
193-205
2008
-
-
2
-
4
-
2
2
-
-
1
6
-
7
-
-
2
-
-
2
6
-
6
-
2
-
1
2
2
-
-
-
1
-
-
-
-
2
-
-
4
-
-
2
1
2
-
-
1
6
-
-
-
2
-
2
6
-
6
-
2
-
1
2
2
-
-
-
-
-
-
-
-
-
680893
Tarze
Extracellular production of hy ...
Escherichia coli
J. Biol. Chem.
282
8759-8767
2007
1
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-
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-
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1
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3
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1
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3
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1
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1
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1
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3
-
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-
680123
Jain
Catalytic and regulatory prope ...
Synechococcus elongatus
Indian J. Exp. Biol.
44
767-772
2006
-
-
-
-
-
-
-
1
-
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1
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1
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8
-
1
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1
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1
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8
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1
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-
664881
Riemenschneider
Isolation and characterization ...
Arabidopsis thaliana
FEBS J.
272
1291-1304
2005
-
-
1
-
-
1
2
1
1
-
5
1
-
4
-
-
1
-
-
-
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-
3
1
-
1
-
1
1
-
-
1
-
2
2
-
-
1
1
-
-
1
2
2
-
1
1
-
5
1
-
-
-
1
-
-
-
-
3
1
-
1
-
1
1
-
-
2
-
-
-
-
-
-
648739
Soutourina
Role of D-cysteine desulfhydra ...
Escherichia coli
J. Biol. Chem.
276
40864-40872
2001
-
-
1
-
-
-
-
2
-
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2
1
-
3
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1
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-
4
-
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2
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1
-
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1
1
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-
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-
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2
-
-
2
1
-
-
-
1
-
-
-
-
4
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
645577
Bagchi
-
Partial purification and regul ...
Phormidium uncinatum, Phormidium uncinatum CU 1462/7
Indian J. Exp. Biol.
35
876-880
1997
-
-
-
-
-
-
-
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-
2
-
3
-
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1
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2
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2
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1
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2
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-
-
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648737
Schuetz
-
Thiol accumulation and cystein ...
Cucurbita pepo
Plant Cell Physiol.
32
733-736
1991
-
-
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3
-
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1
-
1
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2
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1
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1
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2
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1
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34752
Nagasawa
Physiological comparison of D- ...
Escherichia coli, Escherichia coli W3110 / ATCC 27325, Pseudomonas putida, Pseudomonas putida CR 1-1
Arch. Microbiol.
149
413-416
1988
-
-
-
-
-
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2
-
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4
-
32
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12
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4
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12
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648734
Schmidt
-
D-cysteine desulfhydrase from ...
Spinacia oleracea
Methods Enzymol.
143
449-453
1987
1
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4
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2
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1
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1
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1
1
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5
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1
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1
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1
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4
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2
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1
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1
1
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5
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-
648735
Rennenberg
-
Cysteine desulphydrase activit ...
Cucurbita pepo, Nicotiana tabacum
Phytochemistry
26
1583-1589
1987
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-
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4
-
2
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2
-
2
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2
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4
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4
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2
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2
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2
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4
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648731
Nagasawa
D-Cysteine desulfhydrase of Es ...
Citrobacter freundii, Enterobacter cloacae, Escherichia coli, Escherichia coli W3110 / ATCC 27325, Klebsiella pneumoniae
Eur. J. Biochem.
153
541-551
1985
-
-
-
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10
13
-
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4
5
-
33
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1
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2
1
29
1
1
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4
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2
1
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1
1
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1
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10
1
13
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4
5
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1
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2
1
29
1
1
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4
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2
1
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648733
Schmidt
-
A cysteine desulfhydrase speci ...
Chlorella fusca
Z. Naturforsch. C
38 c
428-435
1983
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3
1
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2
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1
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1
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1
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5
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1
1
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1
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1
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1
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5
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1
1
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648732
Schmidt
A cysteine desulfhydrase from ...
Spinacia oleracea
Z. Pflanzenphysiol.
107
295-300
1982
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3
1
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1
1
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1
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1
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1
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1
2
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1
1
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3
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1
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1
1
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1
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1
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1
2
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1
1
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