BRENDA - Enzyme Database show
show all sequences of 4.4.1.15

D-Cysteine desulfhydrase of Escherichia coli. Purification and characterization

Nagasawa, T.; Ishii, T.; Kumagai, H.; Yamada, H.; Eur. J. Biochem. 153, 541-551 (1985)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
AgNO3
-
Escherichia coli
D-penicillamine
-
Escherichia coli
HgCl2
-
Escherichia coli
hydroxylamine
-
Escherichia coli
L-cysteine
mixed type inhibition
Escherichia coli
L-Penicillamine
-
Escherichia coli
additional information
no inhibition with EDTA and substrates
Escherichia coli
p-chloromercuribenzoate
-
Escherichia coli
phenylhydrazine
-
Escherichia coli
Zn2+
-
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.04
-
DL-selenocystine
30°C, pH 8.0, based on the concentration of the D-isomer, Escherichia coli
Escherichia coli
0.11
-
DL-lanthionine
30°C, pH 8.0, based on the concentration of the D-isomer, Escherichia coli
Escherichia coli
0.15
-
D-cysteine
30°C, pH 8.0
Escherichia coli
0.22
-
S-phenyl-D-cysteine
30°C, pH 8.0
Escherichia coli
0.23
-
S-n-propyl-D-cysteine
30°C, pH 8.0
Escherichia coli
0.24
-
S-n-butyl-D-cysteine
30°C, pH 8.0
Escherichia coli
0.27
-
D-Cystine
30°C, pH 8.0
Escherichia coli
0.28
-
S-benzyl-D-cysteine
30°C, pH 8.0
Escherichia coli
0.29
-
D-allocystathionine
30°C, pH 8.0
Escherichia coli
0.42
-
S-ethyl-D-cysteine
30°C, pH 8.0
Escherichia coli
0.5
-
S-methyl-D-cysteine
30°C, pH 8.0
Escherichia coli
0.83
-
S-carboxymethyl-D-cysteine
30°C, pH 8.0
Escherichia coli
0.91
-
3-chloro-D-alanine
30°C, pH 8.0, alpha,beta-elimination
Escherichia coli
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
36500
-
SDS-PAGE
Escherichia coli
67000
-
sedimentation equilibrium centrifugation
Escherichia coli
70000
-
gel filtration
Escherichia coli
74000
-
HPLC gel filtration
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-cysteine + H2O
Escherichia coli
-
sulfide + NH3 + pyruvate
-
Escherichia coli
?
D-cysteine + H2O
Enterobacter cloacae
-
sulfide + NH3 + pyruvate
-
Enterobacter cloacae
?
D-cysteine + H2O
Klebsiella pneumoniae
-
sulfide + NH3 + pyruvate
-
Klebsiella pneumoniae
?
D-cysteine + H2O
Citrobacter freundii
-
sulfide + NH3 + pyruvate
-
Citrobacter freundii
?
D-cysteine + H2O
Escherichia coli W3110 / ATCC 27325
-
sulfide + NH3 + pyruvate
-
Escherichia coli W3110 / ATCC 27325
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Citrobacter freundii
-
-
-
Enterobacter cloacae
-
-
-
Escherichia coli
-
deltatrpED102/F'deltatrpED102
-
Escherichia coli W3110 / ATCC 27325
-
deltatrpED102/F'deltatrpED102
-
Klebsiella pneumoniae
-
-
-
Purification (Commentary)
Commentary
Organism
-
Escherichia coli
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
13
-
purified enzyme, 30°C, pH 8.0, with D-cysteine as substrate
Escherichia coli
56.3
-
purified enzyme, 30°C, pH 8.0, with 3-chloro-D-alanine as substrate
Escherichia coli
Storage Stability
Storage Stability
Organism
-20°C, 10 mM phosphate buffer, pH 7, 0.01 mM pyridoxal phosphate, 0.1 mM DTT, 0.1 mM EDTA 50% glycerol, at least 2 months, no loss of activity
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-chloro-D-alanine + H2O
alpha,beta-elimination, more effective substrate than D-cysteine
648731
Escherichia coli
pyruvate + NH3 + Cl-
-
648731
Escherichia coli
?
3-chloro-D-alanine + H2O
alpha,beta-elimination, more effective substrate than D-cysteine
648731
Escherichia coli W3110 / ATCC 27325
pyruvate + NH3 + Cl-
-
648731
Escherichia coli W3110 / ATCC 27325
?
3-chloro-D-alanine + thioglycolic acid
i.e. mercaptoacetic acid, beta-replacement reaction
648731
Escherichia coli
S-carboxymethyl-D-cysteine
product is further metabolized to form pyruvate
648731
Escherichia coli
?
3-chloro-D-alanine + thioglycolic acid
i.e. mercaptoacetic acid, beta-replacement reaction
648731
Escherichia coli W3110 / ATCC 27325
S-carboxymethyl-D-cysteine
product is further metabolized to form pyruvate
648731
Escherichia coli W3110 / ATCC 27325
?
D-allocystathionine + H2O
-
648731
Escherichia coli
?
-
-
-
?
D-cysteine + H2O
-
648731
Escherichia coli
sulfide + NH3 + pyruvate
-
648731
Escherichia coli
?
D-cysteine + H2O
-
648731
Enterobacter cloacae
sulfide + NH3 + pyruvate
-
648731
Enterobacter cloacae
?
D-cysteine + H2O
-
648731
Klebsiella pneumoniae
sulfide + NH3 + pyruvate
-
648731
Klebsiella pneumoniae
?
D-cysteine + H2O
-
648731
Citrobacter freundii
sulfide + NH3 + pyruvate
-
648731
Citrobacter freundii
?
D-cysteine + H2O
alpha,beta-elimination
648731
Escherichia coli
sulfide + NH3 + pyruvate
-
648731
Escherichia coli
?
D-cysteine + H2O
-
648731
Escherichia coli W3110 / ATCC 27325
sulfide + NH3 + pyruvate
-
648731
Escherichia coli W3110 / ATCC 27325
?
D-cysteine + H2O
alpha,beta-elimination
648731
Escherichia coli W3110 / ATCC 27325
sulfide + NH3 + pyruvate
-
648731
Escherichia coli W3110 / ATCC 27325
?
D-cystine + H2O
-
648731
Escherichia coli
?
-
-
-
?
DL-lanthionine + H2O
-
648731
Escherichia coli
?
-
-
-
?
DL-selenocysteine + H2O
very poor substrate
648731
Escherichia coli
selenide + L-alanine
-
-
-
?
DL-selenocystine + H2O
-
648731
Escherichia coli
?
-
-
-
?
additional information
L-cysteine is not utilized
648731
Escherichia coli
?
-
-
-
-
additional information
L-cysteine is not utilized
648731
Enterobacter cloacae
?
-
-
-
-
additional information
L-cysteine is not utilized
648731
Klebsiella pneumoniae
?
-
-
-
-
additional information
L-cysteine is not utilized
648731
Citrobacter freundii
?
-
-
-
-
additional information
L-cysteine is not utilized
648731
Escherichia coli W3110 / ATCC 27325
?
-
-
-
-
O-methyl-DL-serine + H2O
poor substrate
648731
Escherichia coli
?
-
-
-
?
S-benzyl-D-cysteine + H2O
-
648731
Escherichia coli
benzyl hydrosulfide + pyruvate + NH3
-
-
-
?
S-carboxymethyl-D-cysteine + H2O
-
648731
Escherichia coli
mercaptoacetic acid + pyruvate + NH3
-
-
-
?
S-ethyl-D-cysteine + H2O
-
648731
Escherichia coli
ethanethiol + pyruvate + NH3
-
-
-
?
S-methyl-D-cysteine + H2O
-
648731
Escherichia coli
methanethiol + pyruvate + NH3
-
-
-
?
S-n-butyl-D-cysteine + H2O
-
648731
Escherichia coli
butane-1-thiol + pyruvate + NH3
-
-
-
?
S-n-propyl-D-cysteine + H2O
-
648731
Escherichia coli
propane-1-thiol + pyruvate + NH3
-
-
-
?
S-phenyl-D-cysteine + H2O
-
648731
Escherichia coli
benzenthiol + pyruvate + NH3
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 36500, SDS-PAGE
Escherichia coli
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
45
-
-
Escherichia coli
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
60
-
no loss of activity within 30 min incubation, 0.1 M phosphate buffer, pH 8.0
Escherichia coli
65
-
11% loss of activity within 30 min incubation, 0.1 M phosphate buffer, pH 8.0
Escherichia coli
70
-
24% loss of activity within 30 min incubation, 0.1 M phosphate buffer, pH 8.0
Escherichia coli
75
-
40% loss of activity within 30 min incubation, 0.1 M phosphate buffer, pH 8.0
Escherichia coli
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
alpha,beta-elimination
Escherichia coli
9.5
-
beta-replacement
Escherichia coli
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7
10
-
Escherichia coli
Cofactor
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
2 mol per mol enzyme
Escherichia coli
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.53
-
L-cysteine
-
Escherichia coli
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
2 mol per mol enzyme
Escherichia coli
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
AgNO3
-
Escherichia coli
D-penicillamine
-
Escherichia coli
HgCl2
-
Escherichia coli
hydroxylamine
-
Escherichia coli
L-cysteine
mixed type inhibition
Escherichia coli
L-Penicillamine
-
Escherichia coli
additional information
no inhibition with EDTA and substrates
Escherichia coli
p-chloromercuribenzoate
-
Escherichia coli
phenylhydrazine
-
Escherichia coli
Zn2+
-
Escherichia coli
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.53
-
L-cysteine
-
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.04
-
DL-selenocystine
30°C, pH 8.0, based on the concentration of the D-isomer, Escherichia coli
Escherichia coli
0.11
-
DL-lanthionine
30°C, pH 8.0, based on the concentration of the D-isomer, Escherichia coli
Escherichia coli
0.15
-
D-cysteine
30°C, pH 8.0
Escherichia coli
0.22
-
S-phenyl-D-cysteine
30°C, pH 8.0
Escherichia coli
0.23
-
S-n-propyl-D-cysteine
30°C, pH 8.0
Escherichia coli
0.24
-
S-n-butyl-D-cysteine
30°C, pH 8.0
Escherichia coli
0.27
-
D-Cystine
30°C, pH 8.0
Escherichia coli
0.28
-
S-benzyl-D-cysteine
30°C, pH 8.0
Escherichia coli
0.29
-
D-allocystathionine
30°C, pH 8.0
Escherichia coli
0.42
-
S-ethyl-D-cysteine
30°C, pH 8.0
Escherichia coli
0.5
-
S-methyl-D-cysteine
30°C, pH 8.0
Escherichia coli
0.83
-
S-carboxymethyl-D-cysteine
30°C, pH 8.0
Escherichia coli
0.91
-
3-chloro-D-alanine
30°C, pH 8.0, alpha,beta-elimination
Escherichia coli
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
36500
-
SDS-PAGE
Escherichia coli
67000
-
sedimentation equilibrium centrifugation
Escherichia coli
70000
-
gel filtration
Escherichia coli
74000
-
HPLC gel filtration
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-cysteine + H2O
Escherichia coli
-
sulfide + NH3 + pyruvate
-
Escherichia coli
?
D-cysteine + H2O
Enterobacter cloacae
-
sulfide + NH3 + pyruvate
-
Enterobacter cloacae
?
D-cysteine + H2O
Klebsiella pneumoniae
-
sulfide + NH3 + pyruvate
-
Klebsiella pneumoniae
?
D-cysteine + H2O
Citrobacter freundii
-
sulfide + NH3 + pyruvate
-
Citrobacter freundii
?
D-cysteine + H2O
Escherichia coli W3110 / ATCC 27325
-
sulfide + NH3 + pyruvate
-
Escherichia coli W3110 / ATCC 27325
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
13
-
purified enzyme, 30°C, pH 8.0, with D-cysteine as substrate
Escherichia coli
56.3
-
purified enzyme, 30°C, pH 8.0, with 3-chloro-D-alanine as substrate
Escherichia coli
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, 10 mM phosphate buffer, pH 7, 0.01 mM pyridoxal phosphate, 0.1 mM DTT, 0.1 mM EDTA 50% glycerol, at least 2 months, no loss of activity
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-chloro-D-alanine + H2O
alpha,beta-elimination, more effective substrate than D-cysteine
648731
Escherichia coli
pyruvate + NH3 + Cl-
-
648731
Escherichia coli
?
3-chloro-D-alanine + H2O
alpha,beta-elimination, more effective substrate than D-cysteine
648731
Escherichia coli W3110 / ATCC 27325
pyruvate + NH3 + Cl-
-
648731
Escherichia coli W3110 / ATCC 27325
?
3-chloro-D-alanine + thioglycolic acid
i.e. mercaptoacetic acid, beta-replacement reaction
648731
Escherichia coli
S-carboxymethyl-D-cysteine
product is further metabolized to form pyruvate
648731
Escherichia coli
?
3-chloro-D-alanine + thioglycolic acid
i.e. mercaptoacetic acid, beta-replacement reaction
648731
Escherichia coli W3110 / ATCC 27325
S-carboxymethyl-D-cysteine
product is further metabolized to form pyruvate
648731
Escherichia coli W3110 / ATCC 27325
?
D-allocystathionine + H2O
-
648731
Escherichia coli
?
-
-
-
?
D-cysteine + H2O
-
648731
Escherichia coli
sulfide + NH3 + pyruvate
-
648731
Escherichia coli
?
D-cysteine + H2O
-
648731
Enterobacter cloacae
sulfide + NH3 + pyruvate
-
648731
Enterobacter cloacae
?
D-cysteine + H2O
-
648731
Klebsiella pneumoniae
sulfide + NH3 + pyruvate
-
648731
Klebsiella pneumoniae
?
D-cysteine + H2O
-
648731
Citrobacter freundii
sulfide + NH3 + pyruvate
-
648731
Citrobacter freundii
?
D-cysteine + H2O
alpha,beta-elimination
648731
Escherichia coli
sulfide + NH3 + pyruvate
-
648731
Escherichia coli
?
D-cysteine + H2O
-
648731
Escherichia coli W3110 / ATCC 27325
sulfide + NH3 + pyruvate
-
648731
Escherichia coli W3110 / ATCC 27325
?
D-cysteine + H2O
alpha,beta-elimination
648731
Escherichia coli W3110 / ATCC 27325
sulfide + NH3 + pyruvate
-
648731
Escherichia coli W3110 / ATCC 27325
?
D-cystine + H2O
-
648731
Escherichia coli
?
-
-
-
?
DL-lanthionine + H2O
-
648731
Escherichia coli
?
-
-
-
?
DL-selenocysteine + H2O
very poor substrate
648731
Escherichia coli
selenide + L-alanine
-
-
-
?
DL-selenocystine + H2O
-
648731
Escherichia coli
?
-
-
-
?
additional information
L-cysteine is not utilized
648731
Escherichia coli
?
-
-
-
-
additional information
L-cysteine is not utilized
648731
Enterobacter cloacae
?
-
-
-
-
additional information
L-cysteine is not utilized
648731
Klebsiella pneumoniae
?
-
-
-
-
additional information
L-cysteine is not utilized
648731
Citrobacter freundii
?
-
-
-
-
additional information
L-cysteine is not utilized
648731
Escherichia coli W3110 / ATCC 27325
?
-
-
-
-
O-methyl-DL-serine + H2O
poor substrate
648731
Escherichia coli
?
-
-
-
?
S-benzyl-D-cysteine + H2O
-
648731
Escherichia coli
benzyl hydrosulfide + pyruvate + NH3
-
-
-
?
S-carboxymethyl-D-cysteine + H2O
-
648731
Escherichia coli
mercaptoacetic acid + pyruvate + NH3
-
-
-
?
S-ethyl-D-cysteine + H2O
-
648731
Escherichia coli
ethanethiol + pyruvate + NH3
-
-
-
?
S-methyl-D-cysteine + H2O
-
648731
Escherichia coli
methanethiol + pyruvate + NH3
-
-
-
?
S-n-butyl-D-cysteine + H2O
-
648731
Escherichia coli
butane-1-thiol + pyruvate + NH3
-
-
-
?
S-n-propyl-D-cysteine + H2O
-
648731
Escherichia coli
propane-1-thiol + pyruvate + NH3
-
-
-
?
S-phenyl-D-cysteine + H2O
-
648731
Escherichia coli
benzenthiol + pyruvate + NH3
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 36500, SDS-PAGE
Escherichia coli
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
45
-
-
Escherichia coli
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
60
-
no loss of activity within 30 min incubation, 0.1 M phosphate buffer, pH 8.0
Escherichia coli
65
-
11% loss of activity within 30 min incubation, 0.1 M phosphate buffer, pH 8.0
Escherichia coli
70
-
24% loss of activity within 30 min incubation, 0.1 M phosphate buffer, pH 8.0
Escherichia coli
75
-
40% loss of activity within 30 min incubation, 0.1 M phosphate buffer, pH 8.0
Escherichia coli
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
alpha,beta-elimination
Escherichia coli
9.5
-
beta-replacement
Escherichia coli
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7
10
-
Escherichia coli
Other publictions for EC 4.4.1.15
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746770
Ekimova
Distribution of 1-aminocyclop ...
Methylorubrum extorquens, Methylorubrum extorquens DSM 1338
Antonie van Leeuwenhoek
111
1723-1734
2018
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4
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1
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1
1
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1
1
748473
Li
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Identification of wheat D-cys ...
Triticum aestivum
J. Plant Growth Regul.
37
1175-1184
2018
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