Literature summary for 4.4.1.14 extracted from

Kosugi, Y.; Matsuoka, A.; Higashi, A.; Toyohara, N.; Satoh, S.
2-Aminooxyisobutyric acid inhibits the in vitro activities of both 1-aminocyclopropane-1-carboxylate (ACC) synthase and ACC oxidase in ethylene biosynthetic pathway and prolongs vase life of cut carnation flowers (2014), J. Plant Biol., 57, 218-224.

No PubMed abstract available

Search for more literature for 4.4.1.14

Cloned(Commentary)

Cloned (Comment)	Organism
gene DcACS1, recombinant expression in Escherichia coli	Dianthus caryophyllus

Inhibitors

Inhibitors	Comment	Organism	Structure
2-aminooxyisobutyric acid	75% inhibition at 1.1 mM. The compound's action resembles aminooxyacetic acid. The inhibitor significantly increases the vase life of cut flowers by highly reducing the ethylene production through inhibition of the enzyme	Dianthus caryophyllus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.056	-	S-adenosyl-L-methionine	recombinant enzyme, pH 8.5, 30°C	Dianthus caryophyllus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-adenosyl-L-methionine	Dianthus caryophyllus	-	1-aminocyclopropane-1-carboxylate + methylthioadenosine	-	?

Organism

Organism	UniProt	Comment	Textmining
Dianthus caryophyllus	-	gene DcACS1, several cultivars	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
flower	-	Dianthus caryophyllus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine	-	Dianthus caryophyllus	1-aminocyclopropane-1-carboxylate + methylthioadenosine	-	?

Synonyms

Synonyms	Comment	Organism
ACC synthase	-	Dianthus caryophyllus
ACS	-	Dianthus caryophyllus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Dianthus caryophyllus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.5	-	assay at	Dianthus caryophyllus

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	-	Dianthus caryophyllus

General Information

General Information	Comment	Organism
metabolism	the enzyme is important in ethylene production	Dianthus caryophyllus

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Release 2023.1 (January 2023)