Literature summary for 4.4.1.14 extracted from

Capitani, G.; Eliot, A.C.; Gut, H.; Khomutov, R.M.; Kirsch, J.F.; Grutter, M.G.
Structure of 1-aminocyclopropane-1-carboxylate synthase in complex with an amino-oxy analogue of the substrate: implications for substrate binding (2003), Biochim. Biophys. Acta, 1647, 55-60.

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystal structure of ACC synthase in complex with the substrate analogue [2-(aminooxy)ethyl](5'deoxyadenosin-5'-yl)(methyl)sulfonium at 2.01 A resolution, crystals are obtained with the sitting drop method, 0.001 ml of protein solution, consisting of 20 mg/ml ACC synthase, 10 mM [2-(aminooxy)ethyl](5'deoxyadenosin-5'-yl)(methyl)sulfonium, 50 mM HEPES, pH 7.9, 0.01 mM pyridoxal 5'-phosphate, 1 mM dithiothreitol, is mixed with 0.001 ml of precipitating solution containing 30% 2-methyl-2-4-pentanediol and 50 mM MES, pH 6.5	Malus domestica

Crystallization (Comment)

Organism

crystal structure of ACC synthase in complex with the substrate analogue [2-(aminooxy)ethyl](5'deoxyadenosin-5'-yl)(methyl)sulfonium at 2.01 A resolution, crystals are obtained with the sitting drop method, 0.001 ml of protein solution, consisting of 20 mg/ml ACC synthase, 10 mM [2-(aminooxy)ethyl](5'deoxyadenosin-5'-yl)(methyl)sulfonium, 50 mM HEPES, pH 7.9, 0.01 mM pyridoxal 5'-phosphate, 1 mM dithiothreitol, is mixed with 0.001 ml of precipitating solution containing 30% 2-methyl-2-4-pentanediol and 50 mM MES, pH 6.5

Malus domestica

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-adenosyl-L-methionine	Malus domestica	-	1-aminocyclopropane-1-carboxylate + methylthioadenosine	-	?

Organism

Organism	UniProt	Comment	Textmining
Malus domestica	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant truncated form of ACC synthase	Malus domestica

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine	-	Malus domestica	1-aminocyclopropane-1-carboxylate + methylthioadenosine	-	?

Synonyms

Synonyms	Comment	Organism
ACC synthase	-	Malus domestica

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	-	Malus domestica

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Release 2023.1 (January 2023)