Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21(DE3) | Mimosa pudica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.4 | - |
cystathionine | pH 8.5, 37°C, recombinant enzyme | Mimosa pudica |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | the enzyme sequence contains a signal peptide | Mimosa pudica | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cystathionine + H2O | Mimosa pudica | - |
homocysteine + pyruvate + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mimosa pudica | A0A0M3VI47 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Mimosa pudica | - |
seedling | - |
Mimosa pudica | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cystathionine + H2O | - |
Mimosa pudica | homocysteine + pyruvate + NH3 | - |
? | |
additional information | substrate docking study. The largest attractive force that stabilizes the amino group of the ethyl chain moiety is contributed by Arg433. The negative charge of the carboxyl group in the ethyl chain is balanced by a hydrogen bond between it and amino group of Lys271. The propyl chain moiety of cystathionine is stabilized by the guanidium groups of two residues, Arg179 and Arg122'. Phosphate group of PLP forms two hydrogen bonds between hydroxy hydrogen of Ser268 and guanidium hydrogen of Arg122' | Mimosa pudica | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CBL | - |
Mimosa pudica |
Mp CBL | - |
Mimosa pudica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Mimosa pudica |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
purified recombinant GST-tagged enzyme, pH 8.0, stable up to | Mimosa pudica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
21000 | - |
cystathionine | pH 8.5, 37°C, recombinant enzyme | Mimosa pudica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Mimosa pudica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | dependent on | Mimosa pudica |
General Information | Comment | Organism |
---|---|---|
metabolism | molecular relationship between cystathionine beta-lyase and mimosinase (UniProt ID U6BYK3, EC 3.5.1.61). The recombinant Mp mimosinase degrades both mimosine and cystathionine with a much higher turnover number for mimosine compared with cystathionine, and Mp CBL utilizes only cystathionine as a substrate | Mimosa pudica |
additional information | homology modeling and molecular dynamics simulations of Mp CBL. Active site structure | Mimosa pudica |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8750 | - |
cystathionine | pH 8.5, 37°C, recombinant enzyme | Mimosa pudica |