Cloned (Comment) | Organism |
---|---|
mutant enzymes are expressed in the Escherichia coli KS1000 metC:cat strain | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D116A | mutant with reduced catalytic efficiency | Escherichia coli |
D116N | mutant with reduced catalytic efficiency | Escherichia coli |
R372A | mutant with reduced catalytic efficiency | Escherichia coli |
R372K | mutant with reduced catalytic efficiency | Escherichia coli |
R372L | mutant with reduced catalytic efficiency | Escherichia coli |
R58A | mutant with reduced catalytic efficiency | Escherichia coli |
R58K | mutant with reduced catalytic efficiency | Escherichia coli |
R59A | mutant with reduced catalytic efficiency | Escherichia coli |
R59K | mutant with reduced catalytic efficiency | Escherichia coli |
W340F | mutant with reduced catalytic efficiency | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
aminoethoxyvinylglycine | - |
Escherichia coli | |
N-hydrazinocarbonylmethyl-2-nitrobenzamide | - |
Escherichia coli | |
N-hydrazinocarbonylmethyl-2-trifluoromethylbenzamide | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
L-cystathionine | wild type enzyme, at 25°C | Escherichia coli | |
0.55 | - |
L-cystathionine | mutant enzyme D116N, at 25°C | Escherichia coli | |
0.67 | - |
L-cystathionine | mutant enzyme D116A, at 25°C | Escherichia coli | |
1.02 | - |
L-cystathionine | mutant enzyme R59A, at 25°C | Escherichia coli | |
1.35 | - |
L-cystathionine | mutant enzyme W340F, at 25°C | Escherichia coli | |
1.51 | - |
L-cystathionine | mutant enzyme R59K, at 25°C | Escherichia coli | |
5.1 | - |
L-cystathionine | mutant enzyme R58A, at 25°C | Escherichia coli | |
15 | - |
L-cystathionine | mutant enzyme R372K, at 25°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-cystathionine + H2O | - |
Escherichia coli | L-homocysteine + NH3 + pyruvate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CBL | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.1 | - |
L-cystathionine | mutant enzyme R372K, at 25°C | Escherichia coli | |
9.7 | - |
L-cystathionine | mutant enzyme R58A, at 25°C | Escherichia coli | |
34.1 | - |
L-cystathionine | wild type enzyme, at 25°C | Escherichia coli | |
36.7 | - |
L-cystathionine | mutant enzyme R59K, at 25°C | Escherichia coli | |
37.9 | - |
L-cystathionine | mutant enzyme D116N, at 25°C | Escherichia coli | |
41.9 | - |
L-cystathionine | mutant enzyme D116A, at 25°C | Escherichia coli | |
45.8 | - |
L-cystathionine | mutant enzyme R59A, at 25°C | Escherichia coli | |
79 | - |
L-cystathionine | mutant enzyme W340F, at 25°C | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0019 | - |
aminoethoxyvinylglycine | wild type enzyme, at 25°C | Escherichia coli | |
0.0027 | - |
aminoethoxyvinylglycine | mutant enzyme D116N, at 25°C | Escherichia coli | |
0.0027 | - |
aminoethoxyvinylglycine | mutant enzyme R59A, at 25°C | Escherichia coli | |
0.0039 | - |
aminoethoxyvinylglycine | mutant enzyme D116A, at 25°C | Escherichia coli | |
0.006 | - |
aminoethoxyvinylglycine | mutant enzyme R58A, at 25°C | Escherichia coli | |
0.012 | - |
aminoethoxyvinylglycine | mutant enzyme R59K, at 25°C | Escherichia coli | |
0.04 | - |
aminoethoxyvinylglycine | mutant enzyme W340F, at 25°C | Escherichia coli | |
0.055 | - |
aminoethoxyvinylglycine | mutant enzyme R58K, at 25°C | Escherichia coli | |
9.1 | - |
aminoethoxyvinylglycine | mutant enzyme R372K, at 25°C | Escherichia coli |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00134 | - |
mutant enzyme R59A, at 25°C | Escherichia coli | aminoethoxyvinylglycine | |
0.0017 | - |
wild type enzyme, at 25°C | Escherichia coli | aminoethoxyvinylglycine | |
0.00174 | - |
mutant enzyme R58A, at 25°C | Escherichia coli | aminoethoxyvinylglycine | |
0.0026 | - |
mutant enzyme D116A, at 25°C | Escherichia coli | aminoethoxyvinylglycine | |
0.0033 | - |
mutant enzyme D116N, at 25°C | Escherichia coli | aminoethoxyvinylglycine | |
0.0046 | - |
mutant enzyme R59K, at 25°C | Escherichia coli | aminoethoxyvinylglycine | |
0.023 | - |
mutant enzyme R58K, at 25°C | Escherichia coli | aminoethoxyvinylglycine | |
0.043 | - |
mutant enzyme W340F, at 25°C | Escherichia coli | aminoethoxyvinylglycine | |
3.5 | - |
mutant enzyme R372K, at 25°C | Escherichia coli | aminoethoxyvinylglycine |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00009 | - |
L-cystathionine | mutant enzyme R372A, at 25°C | Escherichia coli | |
0.00023 | - |
L-cystathionine | mutant enzyme R372L, at 25°C | Escherichia coli | |
0.385 | - |
L-cystathionine | mutant enzyme R58K, at 25°C | Escherichia coli | |
0.55 | - |
L-cystathionine | mutant enzyme R372K, at 25°C | Escherichia coli | |
1.92 | - |
L-cystathionine | mutant enzyme R58A, at 25°C | Escherichia coli | |
24 | - |
L-cystathionine | mutant enzyme R59K, at 25°C | Escherichia coli | |
45 | - |
L-cystathionine | mutant enzyme R59A, at 25°C | Escherichia coli | |
59 | - |
L-cystathionine | mutant enzyme W340F, at 25°C | Escherichia coli | |
62 | - |
L-cystathionine | mutant enzyme D116A, at 25°C | Escherichia coli | |
69 | - |
L-cystathionine | mutant enzyme D116N, at 25°C | Escherichia coli | |
190 | - |
L-cystathionine | wild type enzyme, at 25°C | Escherichia coli |