Cloned (Comment) | Organism |
---|---|
gene metC or SH2636, phylogenetic tree and analysis, cloning from strains AX3 and CSC1435, expression of His-tagged enzyme in Escherichia coli strain BL21 | Staphylococcus haemolyticus |
General Stability | Organism |
---|---|
addition of 15% glycerol extends the enzymatic lifetime to several days and permitted freezing of the preparation at -20°C | Staphylococcus haemolyticus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ammonium sulfate | 66% inhibition at 4 mM | Staphylococcus haemolyticus | |
glycine | 73% inhibition at 4 mM | Staphylococcus haemolyticus | |
L-cystathionine | substrate inhibition at concentrations above 6 mM | Staphylococcus haemolyticus | |
L-methionine | 40% inhibition at 6 mM | Staphylococcus haemolyticus | |
L-serine | 86% inhibition at 4 mM | Staphylococcus haemolyticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics, overview | Staphylococcus haemolyticus | |
1.2 | - |
L-cystathionine | pH 8.0, 37°C, purified recombinant enzyme | Staphylococcus haemolyticus | |
1.3 | - |
L-cystathionine | pH 8.5, 37°C, purified recombinant enzyme | Staphylococcus haemolyticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42700 | - |
4 * 46000, recombinant His-tagged enzyme, SDS-PAGE, 4 * 42700, detagged enzyme, SDS-PAGE | Staphylococcus haemolyticus |
46000 | - |
4 * 46000, recombinant His-tagged enzyme, SDS-PAGE, 4 * 42700, detagged enzyme, SDS-PAGE | Staphylococcus haemolyticus |
170000 | - |
recombinant enzyme, gel filtration | Staphylococcus haemolyticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-cystathionine + H2O | Staphylococcus haemolyticus | penultimate step in microbial and plant methionine biosynthesis | L-homocysteine + NH3 + pyruvate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus haemolyticus | - |
strains AX3 and JCSC1435, gene metC or SH2636 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography | Staphylococcus haemolyticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-cystathionine + H2O | penultimate step in microbial and plant methionine biosynthesis | Staphylococcus haemolyticus | L-homocysteine + NH3 + pyruvate | - |
? | |
L-cystathionine + H2O | the enzyme catalyzes the split of the a C-N and of the beta C-S bonds through a beta-elimination | Staphylococcus haemolyticus | L-homocysteine + NH3 + pyruvate | - |
? | |
L-cysteine + H2O | very low activity | Staphylococcus haemolyticus | sulfide + NH3 + pyruvate | - |
? | |
L-methionine + H2O | - |
Staphylococcus haemolyticus | methanethiol + NH3 + 2-oxobutanoate | - |
? | |
additional information | no activity with cysteine, cysteinyl, or cysteinylglycine-S-precursors (S-[1-(2-hydroxymethyl)-1-methylbutyl]-lcysteinylglycine and S-[1-(2-hydroxymethyl)-1-methylbutyl]-l-cysteine) | Staphylococcus haemolyticus | ? | - |
? | |
S-[1-(2-hydroxyethyl)-1-methylbutyl]-L-cysteine + H2O | very low activity | Staphylococcus haemolyticus | sulfanylhexanol + ? | - |
? | |
S-[1-(2-hydroxyethyl)-1-methylbutyl]-L-cysteinylglycine + H2O | very low activity | Staphylococcus haemolyticus | sulfanylhexanol + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 46000, recombinant His-tagged enzyme, SDS-PAGE, 4 * 42700, detagged enzyme, SDS-PAGE | Staphylococcus haemolyticus |
Synonyms | Comment | Organism |
---|---|---|
beta-cystathionase | - |
Staphylococcus haemolyticus |
CBL | - |
Staphylococcus haemolyticus |
More | the enzyme belongs to the large family of cysteine-S-conjugate beta-lyases | Staphylococcus haemolyticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Staphylococcus haemolyticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
reaction with methionine | Staphylococcus haemolyticus |
8.5 | - |
reaction with L-cystathionine | Staphylococcus haemolyticus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | dependent on | Staphylococcus haemolyticus |