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Literature summary for 4.4.1.13 extracted from
- Methionine biosynthesis in higher plants. II. Purification and characterization of cystathionine beta-lyase from spinach chloroplasts (1995), Arch. Biochem. Biophys., 316, 585-595.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| DL-propargylglycine | no inhibition | Spinacia oleracea |  |
| L-alpha-(2-aminoethoxyvinyl)glycine | - |
Spinacia oleracea | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.15 | - |
L-cystathionine | chloroplastic isoenzyme | Spinacia oleracea | |
| 0.34 | - |
L-djenkolate | chloroplastic isoenzyme | Spinacia oleracea | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Spinacia oleracea | 9507 | - |
| cytosol | - |
Spinacia oleracea | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44000 | - |
4 * 44000, chloroplastic isoenzyme, SDS-PAGE | Spinacia oleracea |
| 170000 | - |
chloroplastic isoenzyme, gel filtration | Spinacia oleracea |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cystathionine + H2O | Spinacia oleracea | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Spinacia oleracea | - |
a cytosolic and a chloroplastic isoenzyme | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| chloroplastic isoenzyme | Spinacia oleracea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Spinacia oleracea | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Spinacia oleracea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cystathionine + H2O | - |
Spinacia oleracea | ? | - |
? | |
| L-cystathionine + H2O | - |
Spinacia oleracea | L-homocysteine + NH3 + pyruvate | - |
? | |
| L-djenkolate + H2O | - |
Spinacia oleracea | pyruvate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 44000, chloroplastic isoenzyme, SDS-PAGE | Spinacia oleracea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.3 | 9 | with L-cystathionine or L-djenkolate as substrate | Spinacia oleracea |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 9 | pH 7.5: about 20% of maximal activity, pH 7.5-9.0: optimum | Spinacia oleracea |
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Release 2023.1 (January 2023)
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