Activating Compound | Comment | Organism | Structure |
---|---|---|---|
pyridoxal | is 23% as effective as pyridoxal 5'-phosphate | Spinacia oleracea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) | irreversible | Spinacia oleracea | |
Beta-cyanoalanine | - |
Spinacia oleracea | |
Cys | competitive with L-cystathionine | Spinacia oleracea | |
NEM | irreversible | Spinacia oleracea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
L-cystathionine | - |
Spinacia oleracea | |
0.25 | - |
L-djenkolate | - |
Spinacia oleracea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spinacia oleracea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Spinacia oleracea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Spinacia oleracea | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.111 | - |
- |
Spinacia oleracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-cystathionine + H2O | - |
Spinacia oleracea | L-homocysteine + NH3 + pyruvate | - |
? | |
L-djenkolate + H2O | - |
Spinacia oleracea | pyruvate | - |
? | |
mixed disulfide of L-cysteine and L-homocysteine + H2O | - |
Spinacia oleracea | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.6 | - |
Tris-HCl buffer | Spinacia oleracea |
8.8 | - |
potassium borate buffer | Spinacia oleracea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Spinacia oleracea |