Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
General Stability | Organism |
---|---|
not stable to lyophilization | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3,3,3,-trifluoroalanine | irreversible | Escherichia coli | |
Beta-cyanoalanine | - |
Escherichia coli | |
Cys | competitive with L-cystathionine | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
L-cystathionine | - |
Escherichia coli | |
0.25 | - |
L-cystine | - |
Escherichia coli | |
0.36 | - |
L-djenkolate | - |
Escherichia coli | |
0.83 | - |
L-meso-lanthionine | - |
Escherichia coli | |
4.5 | - |
L-homolanthionine | - |
Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
280000 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
215 | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C or 4°C, 50% glycerol, 4 months, stable | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-cystathionine + H2O | - |
Escherichia coli | L-homocysteine + NH3 + pyruvate | - |
? | |
L-cysteine + H2O | - |
Escherichia coli | ? | - |
? | |
L-cystine + H2O | - |
Escherichia coli | L-thiocysteine + pyruvate + NH3 | - |
? | |
L-djenkolate + H2O | - |
Escherichia coli | pyruvate | - |
? | |
L-homolanthionine + H2O | - |
Escherichia coli | ? | - |
? | |
L-meso-lanthionine + H2O | - |
Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | - |
Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | - |
Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.2 | 9 | pH 7.2: 36% of maximal activity, pH 8.0-9.0: optimum | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | 5.7 mol of pyridoxal 5'-phosphate is bound per mol of enzyme | Escherichia coli | |
pyridoxal 5'-phosphate | Km: 0.005 mM | Escherichia coli |