Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.4.1.13 extracted from

  • Guggenheim, S.
    beta-Cystathionase (Salmonella) (1971), Methods Enzymol., 17B, 439-442.
No PubMed abstract available
Search for more literature for 4.4.1.13

General Stability

General Stability Organism
rapid loss of activity during dialysis Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

Inhibitors Comment Organism Structure
Cys substrate inhibition above 2 mM Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.22
-
 L-cystathionine
-
 Salmonella enterica subsp. enterica serovar Typhimurium
0.29
-
 L-cysteine in presence of 10 mM dithiothreitol Salmonella enterica subsp. enterica serovar Typhimurium
0.8
-
 L-cystine
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
-
 me-A15
-
Salmonella enterica subsp. enterica serovar Typhimurium me-A15
-
 me-A15
-

Purification (Commentary)

Purification (Comment) Organism
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.9
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Storage Stability

Storage Stability Organism
-15°C or 4°C, weeks to months, slow loss of activity Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-cystathionine + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium L-homocysteine + NH3 + pyruvate
-
 ?
L-cystathionine + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium me-A15 L-homocysteine + NH3 + pyruvate
-
 ?
L-cysteine + H2O 7.5% of the activity with cystathionine Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
L-cysteine + H2O 7.5% of the activity with cystathionine Salmonella enterica subsp. enterica serovar Typhimurium me-A15 ?
-
 ?
L-cystine + H2O 87% of the activity with cystathionine Salmonella enterica subsp. enterica serovar Typhimurium L-thiocysteine + pyruvate + NH3
-
 ?
L-cystine + H2O 87% of the activity with cystathionine Salmonella enterica subsp. enterica serovar Typhimurium me-A15 L-thiocysteine + pyruvate + NH3
-
 ?