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Literature summary for 4.4.1.13 extracted from

  • Kamitani, H.; Esaki, N.; Tanaka, H.; Soda, K.
    Thermostable S-alkylcysteine alpha, beta-lyase from a thermophile: purification and properties (1990), Agric. Biol. Chem., 54, 2069-2076.
    View publication on PubMed
Search for more literature for 4.4.1.13

Inhibitors

Inhibitors Comment Organism Structure
HgCl2 57% inhibition at 1 mM Bacillus sp. (in: Bacteria)
hydoxylamine complete inhibition at 1 mM Bacillus sp. (in: Bacteria)
p-chloromercuribenzoate 82% inhibition at 1 mM Bacillus sp. (in: Bacteria)
phenylhydrazine complete inhibition at 1 mM Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.73
-
 S-Benzyl-L-cysteine
-
 Bacillus sp. (in: Bacteria)
2
-
 L-selenodjenkolate
-
 Bacillus sp. (in: Bacteria)
3.6
-
 L-djenkolate
-
 Bacillus sp. (in: Bacteria)
4.6
-
 Se-(methyl)-L-selenocysteine
-
 Bacillus sp. (in: Bacteria)
4.8
-
 L-cysteine
-
 Bacillus sp. (in: Bacteria)
5.1
-
 S-methyl-L-cysteine
-
 Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
 2 * 39000, SDS-PAGE Bacillus sp. (in: Bacteria)
76000
-
 gel filtration Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
 41A
-
Bacillus sp. (in: Bacteria) 41A
-
 41A
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus sp. (in: Bacteria)

Storage Stability

Storage Stability Organism
-20°C, 10 mM potassium phospate buffer, pH 7.2, with 50% glycerol, 0.01 mM pyridoxal 5'-phosphate and 0.01% 2-mercaptoethanol, 1 month without loss of activity Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-cysteine + H2O
-
 Bacillus sp. (in: Bacteria) pyruvate + NH3 + hydrogen sulfide
-
 ?
L-cysteine + H2O
-
 Bacillus sp. (in: Bacteria) 41A pyruvate + NH3 + hydrogen sulfide
-
 ?
L-djenkolate
-
 Bacillus sp. (in: Bacteria) pyruvate + NH3 + S-(mercaptomethyl)cysteine
-
 ?
L-djenkolate
-
 Bacillus sp. (in: Bacteria) 41A pyruvate + NH3 + S-(mercaptomethyl)cysteine
-
 ?
L-Selenodjenkolate
-
 Bacillus sp. (in: Bacteria) Se-(hydroselenomethyl)selenocysteine + pyruvate + NH3
-
 ?
L-Selenodjenkolate
-
 Bacillus sp. (in: Bacteria) 41A Se-(hydroselenomethyl)selenocysteine + pyruvate + NH3
-
 ?
S-benzyl-L-cysteine + H2O
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
S-benzyl-L-cysteine + H2O
-
 Bacillus sp. (in: Bacteria) 41A ?
-
 ?
S-methyl-L-cysteine
-
 Bacillus sp. (in: Bacteria) methyl mercaptane + pyruvate + NH3
-
 ?
S-methyl-L-cysteine
-
 Bacillus sp. (in: Bacteria) 41A methyl mercaptane + pyruvate + NH3
-
 ?
Se-(methyl)-L-selenocysteine
-
 Bacillus sp. (in: Bacteria) HSe(CH3) + pyruvate + NH3
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 39000, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
-
 Bacillus sp. (in: Bacteria)