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Literature summary for 4.4.1.13 extracted from
- S-Alkyl-L-cysteine Lyase (Pseudomonas) (1971), Methods Enzymol., 17B, 470-474.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| S-methyl-L-cysteine | substrate inhibition, when concentration exceeds 10 mM | Pseudomonas cruciviae |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.008 | - |
pyridoxal phosphate | - |
Pseudomonas cruciviae | |
| 2.3 | - |
S-methyl-L-cysteine | - |
Pseudomonas cruciviae | |
| 2.3 | - |
S-methyl-L-cysteine sulfoxide | - |
Pseudomonas cruciviae | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas cruciviae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas cruciviae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, almost all activity lost after two weeks | Pseudomonas cruciviae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-methyl-L-cysteine | - |
Pseudomonas cruciviae | methyl mercaptane + pyruvate + NH3 | - |
ir | |
| S-methyl-L-cysteine sulfoxide | - |
Pseudomonas cruciviae | methyl methanethiolsulfinate + pyruvate + NH3 | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 23 | - |
- |
Pseudomonas cruciviae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.8 | - |
- |
Pseudomonas cruciviae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Pseudomonas cruciviae | |
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Release 2023.1 (January 2023)
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