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Literature summary for 4.4.1.11 extracted from

  • Kudou, D.; Yasuda, E.; Hirai, Y.; Tamura, T.; Inagaki, K.
    Molecular cloning and characterization of L-methionine gamma-lyase from Streptomyces avermitilis (2015), J. Biosci. Bioeng., 120, 380-383 .
    View publication on PubMed
Search for more literature for 4.4.1.11

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Streptomyces avermitilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
 L-cysteine pH 8.0, 37°C Streptomyces avermitilis
1.6
-
 L-methionine pH 8.0, 37°C Streptomyces avermitilis
1.78
-
 L-homocysteine pH 8.0, 37°C Streptomyces avermitilis

Organism

Organism UniProt Comment Textmining
Streptomyces avermitilis Q826W3
-
-
Streptomyces avermitilis DSM 46492 Q826W3
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Streptomyces avermitilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-cysteine + H2O
-
 Streptomyces avermitilis sulfide + NH3 + pyruvate
-
 ?
L-cysteine + H2O
-
 Streptomyces avermitilis DSM 46492 sulfide + NH3 + pyruvate
-
 ?
L-homocysteine + H2O
-
 Streptomyces avermitilis sulfide + NH3 + 2-oxobutanoate
-
 ?
L-homocysteine + H2O
-
 Streptomyces avermitilis DSM 46492 sulfide + NH3 + 2-oxobutanoate
-
 ?
L-methionine + H2O
-
 Streptomyces avermitilis methanethiol + NH3 + 2-oxobutanoate
-
 ?
L-methionine + H2O
-
 Streptomyces avermitilis DSM 46492 methanethiol + NH3 + 2-oxobutanoate
-
 ?

Subunits

Subunits Comment Organism
? x * 42994, calculated from sequence Streptomyces avermitilis

Synonyms

Synonyms Comment Organism
sav7062
-
 Streptomyces avermitilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.03
-
 L-cysteine pH 8.0, 37°C Streptomyces avermitilis
1.7
-
 L-homocysteine pH 8.0, 37°C Streptomyces avermitilis
2.9
-
 L-methionine pH 8.0, 37°C Streptomyces avermitilis

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Streptomyces avermitilis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.15
-
 L-cysteine pH 8.0, 37°C Streptomyces avermitilis
0.94
-
 L-homocysteine pH 8.0, 37°C Streptomyces avermitilis
1.81
-
 L-methionine pH 8.0, 37°C Streptomyces avermitilis