Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.4.1.11 extracted from

  • Foo, T.C.; Terentis, A.C.; Venkatachalam, K.V.
    A continuous spectrophotometric assay and nonlinear kinetic analysis of methionine gamma-lyase catalysis (2016), Anal. Biochem., 507, 21-26 .
    View publication on PubMed
Search for more literature for 4.4.1.11

Application

Application Comment Organism
analysis assay for methionine gamma-lyase-catalyzed gamma-elimination reactions of L-methionine and its analogues with 2-oxobutanoate as product. The assay employs UV-Vis spectrophotometry to continuously monitor the rate of formation of 2-oxobutanoate by its absorbance at 315 nm Porphyromonas gingivalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2
-
 L-methionine pH 7.5, 37°C Porphyromonas gingivalis
38
-
 L-methionine sulfone pH 7.5, 37°C Porphyromonas gingivalis

Organism

Organism UniProt Comment Textmining
Porphyromonas gingivalis C3VMV9
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-methionine + H2O
-
 Porphyromonas gingivalis methanethiol + NH3 + 2-oxobutanoate
-
 ?
L-methionine sulfone + H2O
-
 Porphyromonas gingivalis ? + NH3 + 2-oxobutanoate
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.47
-
 L-methionine pH 7.5, 37°C Porphyromonas gingivalis
34.13
-
 L-methionine sulfone pH 7.5, 37°C Porphyromonas gingivalis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.9
-
 L-methionine sulfone pH 7.5, 37°C Porphyromonas gingivalis
4.55
-
 L-methionine pH 7.5, 37°C Porphyromonas gingivalis