Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | recombinant METase alone inhibits the proliferation of 15/15 NB cell lines in vitro in a dose-dependent manner | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
SDS-PAGE | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
QAE-Sepharose column chromatography | Pseudomonas putida |
Storage Stability | Organism |
---|---|
4°C, 20 mM potassium phosphate buffer (pH 7.4) with 0.02 mM pyridoxal 5'-phosphate, 1 mM EDTA (pH 8.0), 0.01% (v/v) beta-mercaptoethanol containing 0.3 M NaCl, 2 months, enzyme remains stable | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-methionine + H2O | - |
Pseudomonas putida | methanethiol + NH3 + 2-oxobutanoate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
L-methionine gamma-lyase | - |
Pseudomonas putida |
METase | - |
Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Pseudomonas putida |