Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain HB101 | Pseudomonas putida |
Protein Variants | Comment | Organism |
---|---|---|
C116A | the mutant possesses little catalytic activity, while its affinity for each substrate is almost the same as that of the wild type enzyme | Pseudomonas putida |
C116D | the mutant possesses little catalytic activity, while its affinity for each substrate is almost the same as that of the wild type enzyme | Pseudomonas putida |
C116E | the mutant possesses little catalytic activity, while its affinity for each substrate is almost the same as that of the wild type enzyme | Pseudomonas putida |
C116F | the mutant possesses little catalytic activity, while its affinity for each substrate is almost the same as that of the wild type enzyme | Pseudomonas putida |
C116G | the mutant possesses little catalytic activity, while its affinity for each substrate is almost the same as that of the wild type enzyme | Pseudomonas putida |
C116H | the mutant possesses little catalytic activity and shows marked increase in activity toward L-cysteine and a decrease in that toward L-methionine | Pseudomonas putida |
C116I | the mutant possesses little catalytic activity, while its affinity for each substrate is almost the same as that of the wild type enzyme | Pseudomonas putida |
C116K | the mutant possesses little catalytic activity, while its affinity for each substrate is almost the same as that of the wild type enzyme | Pseudomonas putida |
C116L | the mutant possesses little catalytic activity, while its affinity for each substrate is almost the same as that of the wild type enzyme | Pseudomonas putida |
C116M | the mutant possesses little catalytic activity, while its affinity for each substrate is almost the same as that of the wild type enzyme | Pseudomonas putida |
C116N | the mutant possesses little catalytic activity, while its affinity for each substrate is almost the same as that of the wild type enzyme | Pseudomonas putida |
C116P | the mutant possesses little catalytic activity, while its affinity for each substrate is almost the same as that of the wild type enzyme | Pseudomonas putida |
C116Q | the mutant possesses little catalytic activity, while its affinity for each substrate is almost the same as that of the wild type enzyme | Pseudomonas putida |
C116R | the mutant possesses little catalytic activity, while its affinity for each substrate is almost the same as that of the wild type enzyme | Pseudomonas putida |
C116S | the mutant possesses little catalytic activity, while its affinity for each substrate is almost the same as that of the wild type enzyme | Pseudomonas putida |
C116T | the mutant possesses little catalytic activity, while its affinity for each substrate is almost the same as that of the wild type enzyme | Pseudomonas putida |
C116V | the mutant possesses little catalytic activity, while its affinity for each substrate is almost the same as that of the wild type enzyme | Pseudomonas putida |
C116W | the mutant possesses little catalytic activity, while its affinity for each substrate is almost the same as that of the wild type enzyme | Pseudomonas putida |
C116Y | the mutant possesses little catalytic activity, while its affinity for each substrate is almost the same as that of the wild type enzyme | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
propargylglycine | - |
Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
L-methionine | mutant enzyme C116S, at 37°C | Pseudomonas putida | |
0.12 | - |
L-cysteine | mutant enzyme C116S, at 37°C | Pseudomonas putida | |
0.24 | - |
DL-homocysteine | mutant enzyme C116S, at 37°C | Pseudomonas putida | |
0.39 | - |
L-cysteine | mutant enzyme C116A, at 37°C | Pseudomonas putida | |
0.53 | - |
L-cysteine | wild type enzyme, at 37°C | Pseudomonas putida | |
0.63 | - |
L-cysteine | mutant enzyme C116H, at 37°C | Pseudomonas putida | |
0.92 | - |
L-methionine | wild type enzyme, at 37°C | Pseudomonas putida | |
1.42 | - |
L-methionine | mutant enzyme C116H, at 37°C | Pseudomonas putida | |
1.61 | - |
DL-homocysteine | mutant enzyme C116A, at 37°C | Pseudomonas putida | |
1.8 | - |
DL-homocysteine | mutant enzyme C116H, at 37°C | Pseudomonas putida | |
1.82 | - |
DL-homocysteine | wild type enzyme, at 37°C | Pseudomonas putida | |
2.85 | - |
L-methionine | mutant enzyme C116A, at 37°C | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | P13254 | - |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Toyopearl 650M column chromatography and Sephacryl S-200 HR gel filtration | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-chloro-L-alanine + H2O | - |
Pseudomonas putida | ? | - |
? | |
DL-homocysteine + H2O | highest substrate specificity for DL-homocysteine | Pseudomonas putida | hydrogen sulfide + NH3 + 2-oxobutanoate | - |
? | |
L-cysteine + H2O | - |
Pseudomonas putida | sulfide + NH3 + pyruvate | - |
? | |
L-ethionine + H2O | - |
Pseudomonas putida | ethanethiol + NH3 + 2-oxobutanoate | - |
? | |
L-methionine + H2O | - |
Pseudomonas putida | methanethiol + NH3 + 2-oxobutanoate | - |
? | |
additional information | no activity with O-phospho-L-serine | Pseudomonas putida | ? | - |
? | |
O-acetyl-L-serine + H2O | weak activity | Pseudomonas putida | 2-oxopropanoate + NH3 + acetate | - |
? | |
O-succinyl-L-homoserine + H2O | weak activity | Pseudomonas putida | ? | - |
? | |
S-ethyl-L-cysteine + H2O | weak activity | Pseudomonas putida | ethanethiol + NH3 + 2-oxopropanoate | - |
? | |
S-methyl-L-cysteine + H2O | weak activity | Pseudomonas putida | methanethiol + NH3 + 2-oxopropanoate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | - |
Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
L-methionine gamma-lyase | - |
Pseudomonas putida |
MGL | - |
Pseudomonas putida |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
L-methionine | mutant enzyme C116H, at 37°C | Pseudomonas putida | |
0.43 | - |
L-cysteine | mutant enzyme C116A, at 37°C | Pseudomonas putida | |
0.57 | - |
L-cysteine | mutant enzyme C116S, at 37°C | Pseudomonas putida | |
1.46 | - |
L-methionine | mutant enzyme C116A, at 37°C | Pseudomonas putida | |
1.74 | - |
DL-homocysteine | mutant enzyme C116A, at 37°C | Pseudomonas putida | |
1.77 | - |
L-methionine | mutant enzyme C116S, at 37°C | Pseudomonas putida | |
2.27 | - |
L-cysteine | wild type enzyme, at 37°C | Pseudomonas putida | |
2.59 | - |
DL-homocysteine | mutant enzyme C116S, at 37°C | Pseudomonas putida | |
25.39 | - |
L-methionine | wild type enzyme, at 37°C | Pseudomonas putida | |
30 | - |
DL-homocysteine | mutant enzyme C116H, at 37°C | Pseudomonas putida | |
43.8 | - |
L-cysteine | mutant enzyme C116H, at 37°C | Pseudomonas putida | |
44.84 | - |
DL-homocysteine | wild type enzyme, at 37°C | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Pseudomonas putida |