Literature summary for 4.4.1.11 extracted from

Sato, D.; Karaki, T.; Shimizu, A.; Kamei, K.; Harada, S.; Nozaki, T.
Crystallization and preliminary X-ray analysis of L-methionine gamma-lyase 1 from Entamoeba histolytica (2008), Acta Crystallogr. Sect. F, 64, 697-699.

Search for more literature for 4.4.1.11

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 Star (DE3) cells	Entamoeba histolytica

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapour diffusion method, using 1.8 M ammonium sulfate, 0.1 M cacodylate buffer pH 6.2, 0.1 M lithium citrate, and 0.01 M betaine	Entamoeba histolytica

Organism

Organism	UniProt	Comment	Textmining
Entamoeba histolytica	Q86D28	-	-

Purification (Commentary)

Purification (Comment)	Organism
GSTrap HP column chromatography and Sephacryl S-300 gel filtration	Entamoeba histolytica

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-methionine + H2O	-	Entamoeba histolytica	methanethiol + NH3 + 2-oxobutanoate	-	?

Subunits

Subunits	Comment	Organism
tetramer	x-ray crystallography	Entamoeba histolytica

Synonyms

Synonyms	Comment	Organism
L-methionine gamma-lyase 1	-	Entamoeba histolytica
MGL	-	Entamoeba histolytica

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	-	Entamoeba histolytica

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Release 2023.1 (January 2023)