Literature summary for 4.4.1.11 extracted from

Manukhov, I.V.; Mamaeva, D.V.; Rastorguev, S.M.; Faleev, N.G.; Morozova, E.A.; Demidkina, T.V.; Zavilgelsky, G.B.
A gene encoding L-methionine gamma-lyase is present in Enterobacteriaceae family genomes: identification and characterization of Citrobacter freundii L-methionine gamma-lyase (2005), J. Bacteriol., 187, 3889-3893.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Citrobacter freundii

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.16	-	L-cysteine	recombinant enzyme	Citrobacter freundii
0.17	-	S-ethyl-L-cysteine	recombinant enzyme	Citrobacter freundii
0.18	-	L-cysteine	wild-type	Citrobacter freundii
0.18	-	S-Benzyl-L-cysteine	recombinant enzyme	Citrobacter freundii
0.19	-	S-Benzyl-L-cysteine	wild-type	Citrobacter freundii
0.49	-	S-ethyl-L-cysteine	wild-type	Citrobacter freundii
0.54	-	L-ethionine	recombinant enzyme	Citrobacter freundii
0.56	-	L-ethionine	wild-type	Citrobacter freundii
0.61	-	S-methyl-L-cysteine	wild-type	Citrobacter freundii
0.7	-	L-methionine	wild-type	Citrobacter freundii
0.7	-	L-methionine	recombinant enzyme	Citrobacter freundii
0.71	-	S-methyl-L-cysteine	recombinant enzyme	Citrobacter freundii
0.97	-	DL-homocysteine	recombinant enzyme	Citrobacter freundii
1.1	-	DL-homocysteine	wild-type	Citrobacter freundii

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
43000	45000	SDS-PAGE	Citrobacter freundii

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-methionine + H2O	Citrobacter freundii	-	methanethiol + NH3 + 2-oxobutanoate	-	?
L-methionine + H2O	Citrobacter freundii ATCC 29063	-	methanethiol + NH3 + 2-oxobutanoate	-	?

Organism

Organism	UniProt	Comment	Textmining
Citrobacter freundii	Q84AR1	ATCC29063	-
Citrobacter freundii ATCC 29063	Q84AR1	ATCC29063	-

Purification (Commentary)

Purification (Comment)	Organism
-	Citrobacter freundii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
10	-	purified enzyme	Citrobacter freundii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
DL-homocysteine + H2O	-	Citrobacter freundii	hydrogen sulfide + NH3 + 2-oxobutanoate	-	?
DL-homocysteine + H2O	-	Citrobacter freundii ATCC 29063	hydrogen sulfide + NH3 + 2-oxobutanoate	-	?
L-cysteine + H2O	-	Citrobacter freundii	sulfide + NH3 + pyruvate	-	?
L-cysteine + H2O	-	Citrobacter freundii ATCC 29063	sulfide + NH3 + pyruvate	-	?
L-ethionine + H2O	-	Citrobacter freundii	ethanethiol + NH3 + 2-oxobutanoate	-	?
L-ethionine + H2O	-	Citrobacter freundii ATCC 29063	ethanethiol + NH3 + 2-oxobutanoate	-	?
L-methionine + H2O	-	Citrobacter freundii	methanethiol + NH3 + 2-oxobutanoate	-	?
L-methionine + H2O	-	Citrobacter freundii ATCC 29063	methanethiol + NH3 + 2-oxobutanoate	-	?
S-benzyl-L-cysteine + H2O	-	Citrobacter freundii	thiobenzyl alcohol + NH3 + 2-oxopropanoate	-	?
S-benzyl-L-cysteine + H2O	-	Citrobacter freundii ATCC 29063	thiobenzyl alcohol + NH3 + 2-oxopropanoate	-	?
S-ethyl-L-cysteine + H2O	-	Citrobacter freundii	ethanethiol + NH3 + 2-oxopropanoate	-	?
S-methyl-L-cysteine + H2O	-	Citrobacter freundii	methanethiol + NH3 + 2-oxopropanoate	-	?

Synonyms

Synonyms	Comment	Organism
L-methionine gamma-lyase	-	Citrobacter freundii
MGL	-	Citrobacter freundii

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
2.24	-	L-cysteine	wild-type	Citrobacter freundii
2.33	-	L-cysteine	recombinant enzyme	Citrobacter freundii
4.6	-	S-methyl-L-cysteine	recombinant enzyme	Citrobacter freundii
5.03	-	S-ethyl-L-cysteine	recombinant enzyme	Citrobacter freundii
5.1	-	DL-homocysteine	wild-type	Citrobacter freundii
5.97	-	S-methyl-L-cysteine	wild-type	Citrobacter freundii
6.2	-	L-ethionine	wild-type	Citrobacter freundii
6.2	-	L-methionine	recombinant enzyme	Citrobacter freundii
6.5	-	L-methionine	wild-type	Citrobacter freundii
6.78	-	L-ethionine	recombinant enzyme	Citrobacter freundii
6.8	-	S-ethyl-L-cysteine	wild-type	Citrobacter freundii
8.16	-	S-Benzyl-L-cysteine	recombinant enzyme	Citrobacter freundii
8.51	-	DL-homocysteine	recombinant enzyme	Citrobacter freundii
10.9	-	S-Benzyl-L-cysteine	wild-type	Citrobacter freundii

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Release 2023.1 (January 2023)